Concentrations of p-synephrine in fruits and leaves of Citrus species (Rutaceae) and the acute toxicity testing of Citrus aurantium extract and p-synephrine

被引：82

作者：

Arbo, M. D. ^{[1
]}

Larentis, E. R. ^{[1
]}

Linck, V. M. ^{[1
]}

Aboy, A. L. ^{[1
]}

Pimentel, A. L. ^{[2
]}

Henriques, A. T. ^{[1
]}

Dallegrave, E. ^{[3
]}

Garcia, S. C. ^{[4
]}

Leal, M. B. ^{[2
]}

Limberger, R. P. ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, Fac Farm, Programa Posgrad Ciencias Farmaceut, BR-90610000 Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Farmacol, BR-90050170 Porto Alegre, RS, Brazil

[3] Fdn Estadual Prod & Pesquisa Saude, Ctr Informacoes Toxicol Rio Grande Sul, BR-90650090 Porto Alegre, RS, Brazil

[4] Univ Fed Santa Maria, Dept Anal Clin & Toxicol, BR-97110970 Santa Maria, RS, Brazil

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2008年 / 46卷 / 08期

关键词：

synephrine; Citrus; Rutaceae; acute toxicity;

D O I：

10.1016/j.fct.2008.04.037

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Dietary supplements containing bitter orange unripe fruit extract/p-synephrine are consumed worldwide for lose weight. This study were conducted to determine the concentration of p-synephrine in unripe fruits and leaves from Citrus aurantium Lin, C sinensis Osbeck, C. deliciosa Ten, C. limon Burm and C. limonia Osbeck, collected in Southern Brazil, and to evaluate the acute toxicity of C. aurantium extract and p-synephrine. A high performance liquid chromatographic method with diode array detector (HPLC-DAD) was optimized and validated for determination of p-synephrine, The results indicate that all of analyzed samples present p-synephrine in amounts that range from 0.012% to 0.099% in the unripe fruits and 0.029 to 0.438% in the leaves. Acute oral administration of C. aurantium extracts (2.5% p-synephrine, 3005000 mg/kg) in mice produced reduction of locomotor activity, p-synephrine (150-2000 mg/kg) produced piloerection, gasping, salivation, exophtalmia and reduction in locomotor activity, which was confirmed in spontaneous locomotor activity test. All the effects were reversible and persisted for 34 h. The toxic effects observed seem to be related with adrenergic Stimulation and should alert for possible side effects of p-synephrine and C. aurantium. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：2770 / 2775

页数：6

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