A bifunctional DNA probe for sensing pH and microRNA using a nanopore

被引：3
|
作者：
Zhang, Yun [1 ]
Song, Peng [2 ]
Guo, Bingyuan [1 ]
Hao, Wenying [1 ]
Liu, Lei [3 ]
Wu, Hai-Chen [1 ,4 ]
机构：
[1] Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
[2] Gen Hosp Chinese Peoples Liberat Army, Dept Geriatr Oncol, Beijing 100853, Peoples R China
[3] Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China
[4] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
来源：
ANALYST | 2020年 / 145卷 / 21期
基金：
中国国家自然科学基金;
关键词：
BIOMARKER DETECTION; REAL-TIME; SENSORS; GENOME;
D O I：
10.1039/d0an01208d
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We have developed a bifunctional probe based on triplex molecular beacons for the measurement of environmental pH and quantification of microRNA-10b using a nanopore. The probe responds sharply to solution pH changes in the range of 6.0-7.5. The limit of detection for microRNA-10b is as low as 5.0 pM.
引用
收藏
页码：7025 / 7029
页数：5
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