Synergy of microcapsule polysaccharides and Bacillus subtilis on the growth, immunity and resistance of sea cucumber Apostichopus japonicus against Vibrio splendidus infection

被引：13

作者：

Fan, Ying ^{[1
]}

Yu, Xiaoqing ^{[1
]}

Xu, La ^{[1
]}

Wang, Shuxian ^{[1
]}

Ye, Haibin ^{[1
]}

Diao, Jing ^{[1
]}

Yang, Xiusheng ^{[1
]}

Li, Tianbao ^{[1
]}

机构：

[1] Mariculture Inst Shandong Prov, Shandong Prov Key Lab Dis Control Mariculture, Qingdao 266002, Shandong, Peoples R China

来源：

FISHERIES SCIENCE | 2013年 / 79卷 / 05期

关键词：

Apostichopus japonicus; Polysaccharide; Bacillus subtilis; Growth; Immunity; Vibrio splendidus; SHRIMP PENAEUS-MONODON; ASTRAGALUS-MEMBRANACEUS; LITOPENAEUS-VANNAMEI; DISEASE RESISTANCE; SPARUS-AURATA; WHITE SHRIMP; BETA-GLUCAN; PROBIOTICS; COMPLEMENT; PERFORMANCE;

D O I：

10.1007/s12562-013-0644-3

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

A 4-week feeding trial was conducted to determine the effects of different dietary supplements on the growth, immunity and resistance of sea cucumber Apostichopus japonicus against Vibrio splendidus infection. The control group was supplied with blank microcapsules, and Astragalus polysaccharide (APS) microcapsules, tuckahoe polysaccharide (TPS) microcapsules, (APS + TPS) microcapsules, (APS + TPS) microcapsules + Bacillus subtilis, were tested for effects. Coelomic fluid was collected at 7-day intervals to test activities of lysozyme (LSZ), superoxide dismutase (SOD), alkaline phosphatase (AKP), and complement 3 (C3) content. After the feeding trial, the specific growth rate of sea cucumbers fed a diet supplemented with (APS + TPS) microcapsules + B. subtilis was significantly increased (P < 0.05); activities of LSZ, SOD, AKP and C3 content were significantly higher than in other groups (P < 0.05). The challenge test showed that the cumulative mortality of sea cucumbers fed a diet supplemented with (APS + TPS) microcapsules + B. subtilis reduced significantly (P < 0.05). In conclusion, dietary combinations of (APS + TPS) microcapsules + B. subtilis has a potential for use in diet formulations for sea cucumbers to significantly increase growth, immunity and disease resistance against V. splendidus infection.

引用

页码：807 / 814

页数：8