Biological pretreatment of corn stover with ligninolytic enzyme for high efficient enzymatic hydrolysis

被引：70
作者：
Wang, Feng-Qin [1 ]
Xie, Hui [1 ]
Chen, Wei [1 ]
Wang, En-Tao [2 ]
Du, Feng-Guang [3 ]
Song, An-Dong [1 ]
机构：
[1] Henan Agr Univ, Coll Life Sci, Key Lab Enzyme Engn Agr Microbiol, Minist Agr, Zhengzhou 450002, Peoples R China
[2] Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Mexico City 11340, DF, Mexico
[3] Henan Tianguan Grp Co Ltd, State Key Lab Motor Vehicle Biofuel Technol, Nanyang 473000, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2013年 / 144卷
关键词：
Corn stover; Ligninolytic enzyme; Bio-pretreatment; Enzymatic hydrolysis; Cellulase; WHITE-ROT FUNGI; PHANEROCHAETE-CHRYSOSPORIUM; ETHANOL-PRODUCTION; WHEAT-STRAW; RICE STRAW; LIGNOCELLULOSIC BIOMASS; LACCASE DETOXIFICATION; MICROBIAL PRETREATMENT; BAMBOO CULMS; SACCHARIFICATION;
D O I：
10.1016/j.biortech.2013.07.012
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Aiming at increasing the efficiency of transferring corn stover into sugars, a biological pretreatment was developed and investigated in this study. The protocol was characterized by the pretreatment with crude ligninolytic enzymes from Phanerochete chrysosporium and Coridus versicolor to break the lignin structure in corn stover, followed by a washing procedure to eliminate the inhibition of ligninolytic enzyme on cellulase. By a 2 d-pretreatment, sugar yield from corn stover hydrolysis could be increased by 50.2% (up to 323 mg/g) compared with that of the control: X-ray diffractometry and FT-IR analysis revealed that biological pretreatment could partially remove the lignin of corn stover, and consequently enhance the enzymatic hydrolysis efficiency of cellulose and hemeicellulose. In addition, the amount of microbial inhibitors, such as acetic acid and furfural, were much lower in biological pretreatment than that in acid pretreatment. This study provided a promising pretreatment method for biotransformation of corn stovers. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：572 / 578
页数：7
相关论文
共 35 条
[1]   Fungal Pretreatment of Lignocellulose by Phanerochaete chrysosporium to Produce Ethanol From Rice Straw [J].
Bak, Jin Seop ;
Ko, Ja Kyong ;
Choi, In-Geol ;
Park, Yong-Cheol ;
Seo, Jin-Ho ;
Kim, Kyoung Heon .
BIOTECHNOLOGY AND BIOENGINEERING, 2009, 104 (03) :471-482
[2]   Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation pretreatment [J].
Bak, Jin Seop ;
Ko, Ja Kyong ;
Han, Young Hwan ;
Lee, Byung Cheol ;
Choi, In-Geol ;
Kim, Kyoung Heon .
BIORESOURCE TECHNOLOGY, 2009, 100 (03) :1285-1290
[3]  
FAN LT, 1980, BIOTECHNOL BIOENG, V22, P177, DOI 10.1002/bit.260220113
[4]   Phenolic constituents in flax bast tissue and inhibition of cellulase and pectinase [J].
Gamble, GR ;
Snook, ME ;
Henriksson, G ;
Akin, DE .
BIOTECHNOLOGY LETTERS, 2000, 22 (09) :741-746
[5]   STRUCTURAL MODIFICATION OF LIGNOCELLULOSICS BY PRETREATMENTS TO ENHANCE ENZYMATIC-HYDROLYSIS [J].
GHARPURAY, MM ;
LEE, YH ;
FAN, LT .
BIOTECHNOLOGY AND BIOENGINEERING, 1983, 25 (01) :157-172
[6]   Biomass recalcitrance: Engineering plants and enzymes for biofuels production [J].
Himmel, Michael E. ;
Ding, Shi-You ;
Johnson, David K. ;
Adney, William S. ;
Nimlos, Mark R. ;
Brady, John W. ;
Foust, Thomas D. .
SCIENCE, 2007, 315 (5813) :804-807
[7]   Biodegradation and Saccharification of Wood Chips of Pinus strobus and Liriodendron tulipifera by White Rot Fungi [J].
Hwang, Soon-Seok ;
Lee, Sung-Jae ;
Kim, Hee Kyu ;
Ka, Jong-Ok ;
Kim, Kyu-Joong ;
Song, Hong-Gyu .
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 18 (11) :1819-1825
[8]   Laccase detoxification of steam-exploded wheat straw for second generation bioethanol [J].
Jurado, Miguel ;
Prieto, Alicia ;
Martinez-Alcala, Angeles ;
Martinez, Angel T. ;
Jesus Martinez, Maria .
BIORESOURCE TECHNOLOGY, 2009, 100 (24) :6378-6384
[9]   Microbial pretreatment of biomass - Potential for reducing severity of thermochemical biomass pretreatment [J].
Keller, FA ;
Hamilton, JE ;
Nguyen, QA .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2003, 105 (1-3) :27-41
[10]   Pretreatment of corn stover by aqueous ammonia [J].
Kim, TH ;
Kim, JS ;
Sunwoo, C ;
Lee, YY .
BIORESOURCE TECHNOLOGY, 2003, 90 (01) :39-47
1234