Continuous Colorimetric Assay for Acetylcholinesterase and Inhibitor Screening with Gold Nanoparticles

被引：136

作者：

Wang, Ming ^{[1
]}

Gu, Xinggui ^{[1
]}

Zhang, Guanxin ^{[1
]}

Zhang, Deqing ^{[1
]}

Zhu, Daoben ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Organ Solids Lab, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China

来源：

LANGMUIR | 2009年 / 25卷 / 04期

关键词：

SENSITIVE DETECTION; ALZHEIMERS-DISEASE; PROTEIN; SENSOR; VISUALIZATION; SITE;

D O I：

10.1021/la803870v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report herein a new colorimetric assay method for acetylcholinesterase (AChE) activity and its inhibitor screening by making use of the following facts: (1) the aggregation of gold nanoparticles (Au-NPs) results in the red-shift of the plasmon absorption due to interparticle plasmon interactions and (2) AChE can catalyze the hydrolysis of acetylthiocholine into thiocholine which can induce the aggregation of Au-NPs. With this convenient method, the activity of AChE with a concentration as low as 0.6 mU/mL can be assayed. Moreover, this assay method is also useful for screening inhibitors of AChE. Given its simplicity and easy-operation, this method may extend to high-throughput screening of AChE inhibitors and relevant drug discovery.

引用

页码：2504 / 2507

页数：4

共 42 条

[1] Label-free biosensors based on optically responsive nanocomposite layers: Sensitivity and dynamic range [J].

Buecker, P. ;

Trileva, E. ;

Himmelhaus, M. ;

Dahint, R. .

LANGMUIR, 2008, 24 (15) :8229-8239

[2] An electrochemical sensor for pesticide assays based on carbon nanotube-enhanced acetycholinesterase activity [J].

Chen, Haode ;

Zuo, Xiaolei ;

Su, Shao ;

Tang, Zhuzhao ;

Wu, Aibo ;

Song, Shiping ;

Zhang, Dabing ;

Fan, Chunhai .

ANALYST, 2008, 133 (09) :1182-1186

[3] Colorimetric detection of lysozyme based on electrostatic interaction with human serum albumin-modified gold nanoparticles [J].

Chen, Yi-Ming ;

Yu, Cheng-Ju ;

Cheng, Tian-Lu ;

Tseng, Wei-Lung .

LANGMUIR, 2008, 24 (07) :3654-3660

[4] Sensing phosphatase activity by using gold nanoparticles [J].

Choi, Yongdoo ;

Ho, Nan-Hui ;

Tung, Ching-Hsuan .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (05) :707-709

[5] EFFICACY OF MONO-PYRIDINIUM AND BIS-PYRIDINIUM OXIMES VERSUS SOMAN, SARIN AND TABUN POISONING IN MICE [J].

CLEMENT, JG .

FUNDAMENTAL AND APPLIED TOXICOLOGY, 1983, 3 (06) :533-535

[6] Gold nanoparticles: Assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology [J].

Daniel, MC ;

Astruc, D .

CHEMICAL REVIEWS, 2004, 104 (01) :293-346

[7] Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles [J].

Elghanian, R ;

Storhoff, JJ ;

Mucic, RC ;

Letsinger, RL ;

Mirkin, CA .

SCIENCE, 1997, 277 (5329) :1078-1081

[8] A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY [J].

ELLMAN, GL ;

COURTNEY, KD ;

ANDRES, V ;

FEATHERSTONE, RM .

BIOCHEMICAL PHARMACOLOGY, 1961, 7 (02) :88-&

[9] Gold nanoparticle sensor for homocysteine thiolactone-induced protein modification [J].

Gates, Arther T. ;

Fakayode, Sayo O. ;

Lowry, Mark ;

Ganea, Gabriela M. ;

Murugeshu, Abitha ;

Robinson, James W. ;

Strongin, Robert M. ;

Warner, Isiah M. .

LANGMUIR, 2008, 24 (08) :4107-4113

[10] PREPARATION AND CHARACTERIZATION OF AU COLLOID MONOLAYERS [J].

GRABAR, KC ;

FREEMAN, RG ;

HOMMER, MB ;

NATAN, MJ .

ANALYTICAL CHEMISTRY, 1995, 67 (04) :735-743

← 1 2 3 4 5 →