Chloroquine pretreatment inhibits toll-like receptor 3 signaling after stroke

被引：36
作者：
Cui, Guiyun [1 ]
Ye, Xinchun [1 ]
Zuo, Tao [2 ]
Zhao, Hui [3 ]
Zhao, Qiuchen [4 ]
Chen, Wei [1 ,4 ,5 ]
Hua, Fang [1 ]
机构：
[1] Xuzhou Med Coll, Affiliated Hosp, Dept Neurol, Xuzhou 221006, Jiangsu, Peoples R China
[2] Soochow Univ, Affiliated Hosp 1, Dept Clin Med, Suzhou, Jiangsu, Peoples R China
[3] Xuzhou Cent Hosp, Dept Neurol, Xuzhou, Jiangsu, Peoples R China
[4] Xuzhou Med Coll, Dept Clin Med, Xuzhou 221006, Jiangsu, Peoples R China
[5] Tengzhou Cent Peoples Hosp, Dept Neurol, Tengzhou, Shandong, Peoples R China
来源：
NEUROSCIENCE LETTERS | 2013年 / 548卷
基金：
中国国家自然科学基金;
关键词：
Stroke; Inflammatory response; TLR3; IFR3; IFN-beta; Chloroquine; TYPE-1; DIABETIC-RATS; ISCHEMIC-STROKE; INJURY; ISCHEMIA/REPERFUSION; ACTIVATION; CYTOKINES; DEATH;
D O I：
10.1016/j.neulet.2013.02.072
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Toll-like receptors (TLRs) mediated signaling is mainly implicated in inflammatory activation which contributes to the initiation and progression of stroke. Using a model of transient global cerebral ischemia (tGCI) in rats, we investigated the changes of pro-inflammation mediators and tested the effects of Chloroquine pretreatment on the expression of pro-inflammation mediators after stroke. Adult Male Sprague-Dawley (SD) rats were subjected to transient global cerebral ischemia (tGCI) and treated without or with Chiloquin pretreatment (60 mg/kg) 2 h before tGCI. Short-term spatial memory capacity, Western blot assay and semi-quantitive RT-PCR were performed. Compared to sham operated rats, tGCI rats showed worsened learning and memory capacity and increased expression of TLR3, interferon regulatory factor 3 (IRF3), and interferon beta (IFN-beta) in the Hippocampus after stroke. Chloroquine pretreatment significantly enhanced rats' short-term spatial memory capacity and attenuated the expression of TLR3, IFR3, and IFN-beta in the Hippocampus compared to non-treatment control in tGCI rats. Therefore, Chloroquine pretreatment of stroke inhibits inflammatory response and improves short-term spatial memory capacity. The TLR3/IFR3-IFN-beta signaling pathway may contribute to the reduced inflammatory response after stroke. Chloroquine warrants further investigation as a therapeutic agent for the treatment of stroke. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：101 / 104
页数：4
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