Low-intensity pulsed ultrasound-mediated stimulation of hematopoietic stem/progenitor cell viability, proliferation and differentiation in vitro

被引：41

作者：

Xu, Peng ^{[1
,2
]}

Gul-Uludag, Hilal ^{[1
,3
,4
]}

Ang, Woon T. ^{[4
]}

Yang, Xiaoyan ^{[1
]}

Huang, Min ^{[1
]}

Marquez-Curtis, Leah ^{[5
]}

McGann, Locksley ^{[5
]}

Janowska-Wieczorek, Anna ^{[6
]}

Xing, James ^{[4
,7
]}

Swanson, Eric ^{[8
]}

Chen, Jie ^{[1
,2
,3
]}

机构：

[1] Univ Alberta, Dept Biomed Engn, ECERF W6 019, Edmonton, AB T6G 2V4, Canada

[2] Natl Inst Nanotechnol, Edmonton, AB T6G 2M9, Canada

[3] Univ Alberta, Dept Elect & Comp Engn, Edmonton, AB T6G 2V4, Canada

[4] IntelligentNano Inc, Edmonton, AB T6G 2M9, Canada

[5] Canadian Blood Serv R&D, Edmonton, AB T6G 2R8, Canada

[6] Univ Alberta, Dept Med, Edmonton, AB T6G 2R8, Canada

[7] Cross Canc Inst, Edmonton, AB T6G 1Z2, Canada

[8] Natl Res Council Canada IRAP, Edmonton, AB T6N 1E4, Canada

来源：

BIOTECHNOLOGY LETTERS | 2012年 / 34卷 / 10期

基金：

加拿大健康研究院; 加拿大自然科学与工程研究理事会;

关键词：

CD34(+) cells; Cord blood; Low-intensity pulsed ultrasound; Peripheral blood leukapheresis product; Stem cell therapy; STEM-CELL; THERAPEUTIC ULTRASOUND; FIBROBLASTS; EXPRESSION; GROWTH;

D O I：

10.1007/s10529-012-0984-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Low-intensity pulsed ultrasound (LIPUS) stimulated the viability, proliferation and differentiation of hematopoietic stem/progenitor cells (HSPC) from fresh and cryopreserved peripheral blood leukapheresis product, as well as cord blood when applied for 10 min each day for 4 days. Cell viability, proliferation and differentiation were assessed on day 5 by viable cell counting, MTS proliferation assay, flow cytometry, and colony-forming unit assay. LIPUS stimulation: (i) enhanced the proliferation of fresh HSPC and maintained the viability of cryopreserved HSPC in vitro; (ii) did not affect the percentage of CD34(+) and CD14(+) cells; and (iii) enhanced burst-forming unit-erythroid colony formation. Hence, we suggest that this novel LIPUS stimulation approach might enhance the efficacy of clinical transplantation and cellular therapies using HSPC.

引用

页码：1965 / 1973

页数：9

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