The Cryptococcus neoformans capsule: lessons from the use of optical tweezers and other biophysical tools

被引：11
|
作者：
Pontes, Bruno [1 ]
Frases, Susana [2 ]
机构：
[1] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Lab Pincas Ot, Coordenacao Programas Estudos Avancados, BR-21941902 Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestrut Celular Hertha Meyer, BR-21941902 Rio De Janeiro, Brazil
来源：
FRONTIERS IN MICROBIOLOGY | 2015年 / 6卷
关键词：
Cryptococcus neoformans; polysaccharide; capsule; optical tweezers; light scattering; viscosity; zeta potential; SINGLE-MOLECULE; POLYSACCHARIDE BIOSYNTHESIS; MONOCLONAL-ANTIBODIES; PASSIVE-IMMUNIZATION; ELASTIC PROPERTIES; GENE-EXPRESSION; GLUCURONOXYLOMANNAN; VIRULENCE; MELANIN; INFECTION;
D O I：
10.3389/fmicb.2015.00640
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The fungal pathogen Cryptococcus neoformans causes life-threatening infections in immunocompromised individuals, representing one of the leading causes of morbidity and mortality in AIDS patients. The main virulence factor of C. neoformans is the polysaccharide capsule; however, many fundamental aspects of capsule structure and function remain poorly understood. Recently, important capsule properties were uncovered using optical tweezers and other biophysical techniques, including dynamic and static light scattering, zeta potential and viscosity analysis. This review provides an overview of the latest findings in this emerging field, explaining the impact of these findings on our understanding of C. neoformans biology and resistance to host immune defenses.
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页数：7
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