Hsa_circ_0029589 knockdown inhibits the proliferation, migration and invasion of vascular smooth muscle cells via regulating miR-214-3p and STIM1

被引：31

作者：

Huang, Zhen ^{[1
]}

Li, Penglei ^{[2
]}

Wu, Leiming ^{[1
]}

Zhang, Dianhong ^{[1
]}

Du, Binbin ^{[1
]}

Liang, Cui ^{[1
]}

Gao, Lu ^{[1
]}

Zhang, Yanzhou ^{[1
]}

Yao, Rui ^{[1
]}

机构：

[1] Zhengzhou Univ, Cardiovasc Hosp, Affiliated Hosp 1, 1 East Jianshe Rd, Zhengzhou 450052, Henan, Peoples R China

[2] Peoples Hosp Zhongmu, Dept Vasculocardiol, Zhengzhou, Henan, Peoples R China

来源：

LIFE SCIENCES | 2020年 / 259卷

基金：

中国国家自然科学基金;

关键词：

Atherosclerosis; Ox-LDL; Vascular smooth muscle cells; hsa_circ_0029589; miR-214-3p; STIM1; LOW-DENSITY; DNA-DAMAGE; ATHEROSCLEROSIS; EXPRESSION; PROMOTES; DISEASE; LDL;

D O I：

10.1016/j.lfs.2020.118251

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Aims: Circular RNAs (circRNAs) are relevant to atherosclerosis progression. However, the role and mechanism of circRNA hsa_circ_0029589 (circ_0029589) in atherosclerosis are not fully understood. This research aims to explore the function and mechanism of circ_0029589 in oxidized low-density lipoprotein (ox-LDL)-caused vas-cular smooth muscle cells (VSMCs) injury in vitro. Main methods: VSMCs were challenged via ox-LDL to mimic atherosclerosis-like injury in vitro. Circ_0029589, microRNA-214-3p (miR-214-3p) and stromal interaction molecule 1 (STIM1) abundances were detected via quantitative reverse transcription polymerase chain reaction or western blot. Cell proliferation was investigated via cell viability, cycle, apoptosis and proliferation-associated protein levels. Cell migration and invasion were assessed via transwell analysis. The relationship between miR-214-3p and circ_0029589 or STIM1 was tested via dual-luciferase reporter analysis and RNA immunoprecipitation. Key findings: Circ_0029589 level was enhanced in ox-LDL-challenged VSMCs. Circ_0029589 interference constrained cell proliferation, migration and invasion in ox-LDL-challenged VSMCs. miR-214-3p was targeted by circ_0029589 and miR-214-3p knockdown weakened the suppressive function of circ_0029589 silence on VSMCs proliferation, migration and invasion. STIM1 was targeted via miR-214-3p and miR-214-3p could suppress VSMCs proliferation, migration and invasion via decreasing STIM1. Moreover, circ_0029589 modulated STIM1 level by miR-214-3p. Significance: Circ_0029589 knockdown repressed proliferation, migration and invasion of VSMCs challenged via ox-LDL by regulating miR-214-3p and STIM1, indicating that circ_0029589 might play important role in atherosclerosis.

引用

页数：9

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