Isolation and identification of crude oil-degrading strain Stenotrophomous maltophilia H9-3

To isolate an efficient hydrocarbon-degrading bacterium strain in use of native bioremediation,Soil in Daqing oil field was collected which had been contaminated with crude oil for more than ten years. The bacteria from the soil were cultured in the enrichment medium containing crude oil and mineral salts.The strains which could display Blood-plate hemolysis were picked out as the target strains with possibility to create biosurfactant. Then by the same way, the target strains were purified for more than three times and named H9-3 which also was identified to Stenotrophomous maltophilia by using 16S rDNA sequence. The strain could also lower the surface tension of its nutrient fluid. It has been concluded that Stenotrophomnas maltophilia H9-3 can live on crued oil as the sole carbon source and has the potential to produce biosuefactants.Finally, crude oil's degradation efficiency of H9-3 was determined to 15.9% by the use of quantifying the lose weight of the crude oil.