Isolation and pathogenicity of pathogens from skin ulceration disease and viscera ejection syndrome of the sea cucumber Apostichopus japonicuss

被引：211

作者：

Deng, Huan ^{[1
]}

He, Chongbo ^{[1
]}

Zhou, Zunchun ^{[1
]}

Liu, Chang ^{[2
]}

Tan, Kefei ^{[1
]}

Wang, Nianbin ^{[1
]}

Jiang, Bei ^{[1
]}

Gao, Xianggang ^{[1
]}

Liu, Weidong ^{[1
]}

机构：

[1] Liaoning Open Lab Appl Marine Biotechnol, Liaoning Ocean & Fisheries Sci Res Inst, Liaoning Key Lab Marine Fishery Mol Biol, Dalian 116023, Peoples R China

[2] China Med Univ, Shenyang 110001, Peoples R China

来源：

AQUACULTURE | 2009年 / 287卷 / 1-2期

关键词：

Sea cucumber; Apostichopus japonicus; Skin ulceration disease; Viscera ejection syndrome; Pathogen; Pathogenicity; SP-NOV; VIBRIO-SPLENDIDUS; COMMUNITIES; SEDIMENTS; BACTERIA; SELENKA; LARVAE;

D O I：

10.1016/j.aquaculture.2008.10.015

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The diseases of cultured sea cucumber have been the bottle-neck for the Sustainable development of sea cucumber (Apostichopus jpaonicus) cultivation in China. Two diseases that Occurred from 2004 and caused mass mortalities of sea cucumbers are still prevalent in Liaoning and Shandong provinces in China. One is skill ulceration syndrome and the other is viscera rejection syndrome. The epidemiology and pathogenicity of the diseases were investigated. Microscopic observations were made oil healthy and diseased individuals of sea cucumbers to record the bacteria, mold and parasites on the surface of body wall. and the relationship of microbes between skill ulceration disease and viscera ejection disease. The two diseases expressed several differences. Six predominant isolates of bacteria (2004A, 2004B, 04101, 04102, 04103 and 04104) were isolated from the sea Cucumbers Suffering from skin ulceration in indoor ponds for wintering and outdoor ponds for Culturing. Two isolates of bacteria (041201 and 041202) were isolated from the sea cucumbers suffering from viscera ejection syndrome in indoor wintering ponds. Induced infection experiments with these isolates of bacteria showed that all eight predominant isolates of bacteria were pathogenic as they showed the same signs as naturally infected and re-isolates from diseased sea Cucumber had the same morphological characteristics. Physiological and biochemical tests and the sequence similarity analysis of the 16S rRNA genes of these bacterial isolates indicated that isolate 2004A was similar to Vibrio cyclitrophicus, 2004B similar to V. splendidus, isolates 04101 and 04103 similar to V. harveyi, 04102 similar to V. tasmaniensis, isolate 04104 similar to Photobacteriurn sp. isolate 041201 similar to Arthrobacter protophormiae, and isolate 041202 similar to Staphylococcus equorum. Antibiotic sensitive test showed that the eight isolates of bacteria were sensitive to Ofloxacin, Levofloxacin hydrochloride, Cefobid. Doxycycline, Novobiocin, but not sensitive to Oxacillin sodium, Aminobenzylpenicillin, Spectinomycin, or Cefradine. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：18 / 27

页数：10

共 39 条

[1]

[Anonymous], 2001, CHINESE HERBAL MED

[2] The skin ulceration disease in cultivated juveniles of Holothuria scabra (Holothuroidea, Echinodermata) [J].

Becker, P ;

Gillan, D ;

Lanterbecq, D ;

Jangoux, M ;

Rasolofonirina, R ;

Rakotovao, J ;

Eeckhaut, I .

AQUACULTURE, 2004, 242 (1-4) :13-30

[3]

Chang Y., 2004, Biology and Aquaculture of Sea Cucumbers and Sea Urchins

[4] Development of anti-reflection (AR) coating on plastic panels for display applications [J].

Chen, DG ;

Yan, YG ;

Westenberg, E ;

Niebauer, D ;

Sakaitani, N ;

Chaudhuri, SR ;

Sato, Y ;

Takamatsu, M .

JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY, 2000, 19 (1-3) :77-82

[5]

Chen J., 2003, SPC BECHE DE MER INF, V18

[6]

Deng Huan, 2005, Fisheries Science (Liaoning), V24, P40

[7]

Deng Huan, 2008, Virologica Sinica, V23, P63, DOI 10.1007/s12250-008-2863-9

[8]

Dong X.Z., 2001, MANUAL SYSTEM IDENTI

[9]

DONG Y, 2005, FISHERIES SCI, V24, P4

[10]

Engelen B, 1998, APPL ENVIRON MICROB, V64, P2814

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