A census of RND superfamily proteins in the Burkholderia genus

被引：14
作者：
Perrin, Elena [1 ]
Fondi, Marco [1 ,2 ]
Papaleo, Maria Cristiana [1 ]
Maida, Isabel [1 ]
Emiliani, Giovanni [3 ]
Buroni, Silvia [4 ]
Pasca, Maria Rosalia [4 ]
Riccardi, Giovanna [4 ]
Fani, Renato [1 ]
机构：
[1] Univ Florence, Dept Biol, Lab Mol & Microbial Evolut, I-50019 Sesto Fiorentino, Fi, Italy
[2] Univ Cambridge, Comp Lab, Cambridge CB2 3QG, England
[3] CNR, Trees & Timber Inst, I-50019 Florence, Italy
[4] Univ Pavia, Dept Biol & Biotechnol Lazzaro Spallanzani, I-27100 Pavia, Italy
来源：
FUTURE MICROBIOLOGY | 2013年 / 8卷 / 07期
关键词：
antibiotic resistance; Burkholderia; efflux pumps; genomics; new antibiotics; RND; MULTIPLE SEQUENCE ALIGNMENT; MEDIATED DRUG-RESISTANCE; MEMBRANE-FUSION PROTEIN; MULTIDRUG EFFLUX PUMP; CRYSTAL-STRUCTURE; PSEUDOMONAS-AERUGINOSA; CEPACIA COMPLEX; ANTIMICROBIAL RESISTANCE; SUBSTRATE-SPECIFICITY; ESCHERICHIA-COLI;
D O I：
10.2217/fmb.13.50
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: The aim of this work was to analyze the eight resistance-nodulation-cell division (RND) families (a group of proteins mainly involved in multidrug resistance of Gram-negative bacteria) in 26 Burkholderia genomes in order to gain knowledge regarding their presence and distribution, to obtain a platform for future experimental tests aimed to identify new molecular targets to be used in antimicrobial therapy against Burkholderia species and to refine the annotation of RND-like sequences in these genomes. Materials & methods: A total of 417 coding sequences were retrieved and analyzed using different bioinformatics tools. Results & conclusion: A complex pattern of RND presence and distribution in the different Burkholderia species was disclosed and a core of proteins represented in all 26 genomes was identified. These core' proteins might represent useful targets of new synthetic antimicrobial compounds. Furthermore, the annotation of RND-like sequences in Burkholderia was refined.
引用
收藏
页码：923 / 937
页数：15
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