A census of RND superfamily proteins in the Burkholderia genus

被引：14

作者：

Perrin, Elena ^{[1
]}

Fondi, Marco ^{[1
,2
]}

Papaleo, Maria Cristiana ^{[1
]}

Maida, Isabel ^{[1
]}

Emiliani, Giovanni ^{[3
]}

Buroni, Silvia ^{[4
]}

Pasca, Maria Rosalia ^{[4
]}

Riccardi, Giovanna ^{[4
]}

Fani, Renato ^{[1
]}

机构：

[1] Univ Florence, Dept Biol, Lab Mol & Microbial Evolut, I-50019 Sesto Fiorentino, Fi, Italy

[2] Univ Cambridge, Comp Lab, Cambridge CB2 3QG, England

[3] CNR, Trees & Timber Inst, I-50019 Florence, Italy

[4] Univ Pavia, Dept Biol & Biotechnol Lazzaro Spallanzani, I-27100 Pavia, Italy

来源：

FUTURE MICROBIOLOGY | 2013年 / 8卷 / 07期

关键词：

antibiotic resistance; Burkholderia; efflux pumps; genomics; new antibiotics; RND; MULTIPLE SEQUENCE ALIGNMENT; MEDIATED DRUG-RESISTANCE; MEMBRANE-FUSION PROTEIN; MULTIDRUG EFFLUX PUMP; CRYSTAL-STRUCTURE; PSEUDOMONAS-AERUGINOSA; CEPACIA COMPLEX; ANTIMICROBIAL RESISTANCE; SUBSTRATE-SPECIFICITY; ESCHERICHIA-COLI;

D O I：

10.2217/fmb.13.50

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aim: The aim of this work was to analyze the eight resistance-nodulation-cell division (RND) families (a group of proteins mainly involved in multidrug resistance of Gram-negative bacteria) in 26 Burkholderia genomes in order to gain knowledge regarding their presence and distribution, to obtain a platform for future experimental tests aimed to identify new molecular targets to be used in antimicrobial therapy against Burkholderia species and to refine the annotation of RND-like sequences in these genomes. Materials & methods: A total of 417 coding sequences were retrieved and analyzed using different bioinformatics tools. Results & conclusion: A complex pattern of RND presence and distribution in the different Burkholderia species was disclosed and a core of proteins represented in all 26 genomes was identified. These core' proteins might represent useful targets of new synthetic antimicrobial compounds. Furthermore, the annotation of RND-like sequences in Burkholderia was refined.

引用

页码：923 / 937

页数：15

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