Statistical breakage of single protein A-IgG bonds reveals crossover from spontaneous to force-induced bond dissociation

被引：50

作者：

Simson, DA ^{[1
]}

Strigl, M ^{[1
]}

Hohenadl, M ^{[1
]}

Merkel, R ^{[1
]}

机构：

[1] Tech Univ Munich, Lehrstuhl Biophys E22, D-85748 Garching, Germany

来源：

PHYSICAL REVIEW LETTERS | 1999年 / 83卷 / 03期

关键词：

D O I：

10.1103/PhysRevLett.83.652

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

Dynamic force spectroscopy was applied to single specific bonds between immunoglobulins of type G and protein A, a staphylococcal receptor or IgG. The resulting spectra of yield forces indicated the crossover from force induced to spontaneous bond dissociation. Moreover, failure of unloaded bonds was observed directly. Extrapolation to vanishing loading rate and direct observation yielded coinciding results.

引用

页码：652 / 655

页数：4

共 19 条

[1] LIFETIME OF THE P-SELECTIN-CARBOHYDRATE BOND AND ITS RESPONSE TO TENSILE FORCE IN HYDRODYNAMIC FLOW [J].

ALON, R ;

HAMMER, DA ;

SPRINGER, TA .

NATURE, 1995, 374 (6522) :539-542

[2]

BELL GI, 1978, SCIENCE, V200, P618, DOI 10.1126/science.347575

[3] Dynamic strength of molecular adhesion bonds [J].

Evans, E ;

Ritchie, K .

BIOPHYSICAL JOURNAL, 1997, 72 (04) :1541-1555

[4] SENSITIVE FORCE TECHNIQUE TO PROBE MOLECULAR ADHESION AND STRUCTURAL LINKAGES AT BIOLOGICAL INTERFACES [J].

EVANS, E ;

RITCHIE, K ;

MERKEL, R .

BIOPHYSICAL JOURNAL, 1995, 68 (06) :2580-2587

[5] ADHESION FORCES BETWEEN INDIVIDUAL LIGAND-RECEPTOR PAIRS [J].

FLORIN, EL ;

MOY, VT ;

GAUB, HE .

SCIENCE, 1994, 264 (5157) :415-417

[6] IMMUNOGLOBULIN BINDING SPECIFICITIES OF THE HOMOLOGY REGIONS (DOMAINS) OF PROTEIN-A [J].

IBRAHIM, S .

SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1993, 38 (04) :368-374

[7] Molecular dynamics study of unbinding of the avidin-biotin complex [J].

Izrailev, S ;

Stepaniants, S ;

Balsera, M ;

Oono, Y ;

Schulten, K .

BIOPHYSICAL JOURNAL, 1997, 72 (04) :1568-1581

[8] IMMOBILIZATION OF PROTEINS TO A CARBOXYMETHYLDEXTRAN-MODIFIED GOLD SURFACE FOR BIOSPECIFIC INTERACTION ANALYSIS IN SURFACE-PLASMON RESONANCE SENSORS [J].

JOHNSSON, B ;

LOFAS, S ;

LINDQUIST, G .

ANALYTICAL BIOCHEMISTRY, 1991, 198 (02) :268-277

[9] FORCE MEASUREMENTS BY MICROMANIPULATION OF A SINGLE ACTIN FILAMENT BY GLASS NEEDLES [J].

KISHINO, A ;

YANAGIDA, T .

NATURE, 1988, 334 (6177) :74-76

[10] LONG-RANGE ATTRACTION AND MOLECULAR-REARRANGEMENTS IN RECEPTOR-LIGAND INTERACTIONS [J].

LECKBAND, DE ;

ISRAELACHVILI, JN ;

SCHMITT, FJ ;

KNOLL, W .

SCIENCE, 1992, 255 (5050) :1419-1421

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