Rapid Point-of-Care Assay by SERS Detection of SARS-CoV-2 Virus and Its Variants

被引：36

作者：

Guan, Peng-Cheng ^{[1
]}

Zhang, Hong ^{[2
]}

Li, Zhi-Yong ^{[1
]}

Xu, Shan-Shan ^{[1
]}

Sun, Miao ^{[1
]}

Tian, Xian-Min ^{[2
]}

Lin, Jia-Sheng ^{[1
]}

Wen, Huan ^{[1
]}

Zhang, Fan-Li ^{[3
]}

Zhang, Yue-Jiao ^{[1
]}

Ma, Zhan ^{[2
]}

Yu, Guang-Jun ^{[2
]}

Yang, Chaoyong ^{[1
]}

Wang, Zhan-Xiang ^{[1
]}

Song, Yanling ^{[1
]}

Li, Jian-Feng ^{[1
]}

Gu, Man-Man ^{[3
]}

机构：

[1] Xiamen Univ, Coll Energy, Coll Mat, State Key Lab Phys Chem Solid Surfaces,Affiliated, Xiamen 361005, Peoples R China

[2] Shanghai Jiao Tong Univ, Shanghai Childrens Hosp, Shanghai 200062, Peoples R China

[3] China Jiliang Univ, Dept Opt & Elect Technol, Hangzhou 310018, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2022年 / 94卷 / 51期

基金：

中国国家自然科学基金;

关键词：

INFECTION; SPIKE;

D O I：

10.1021/acs.analchem.2c03437

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Addressing the spread of coronavirus disease 2019 (COVID-19) has highlighted the need for rapid, accurate, and low-cost diagnostic methods that detect specific antigens for SARS-CoV-2 infection. Tests for COVID-19 are based on reverse transcription PCR (RT-PCR), which requires laboratory services and is time-consuming. Here, by targeting the SARS-CoV-2 spike protein, we present a point-of-care SERS detection platform that specifically detects SARS-CoV-2 antigen in one step by captureing substrates and detection probes based on aptamer-specific recognition. Using the pseudovirus, without any pretreatment, the SARS-CoV-2 virus and its variants were detected by a handheld Raman spectrometer within 5 min. The limit of detection (LoD) for the pseudovirus was 124 TU mu L-1 (18 fM spike protein), with a linear range of 250-10,000 TU mu L-1. Moreover, this assay can specifically recognize the SARS-CoV-2 antigen without cross reacting with specific antigens of other coronaviruses or influenza A. Therefore, the platform has great potential for application in rapid point-of-care diagnostic assays for SARS-CoV-2.

引用

页码：17795 / 17802

页数：8

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