Haemagglutinin displayed on the surface of Lactococcus lactis confers broad cross-clade protection against different H5N1 viruses in chickens

被引：3
作者：
Lei, Han [1 ]
Gao, Tong [1 ]
Cen, Qianhong [1 ]
Peng, Xiaojue [2 ]
机构：
[1] Southwest Jiaotong Univ, Coll Med, Chengdu 610031, Sichuan, Peoples R China
[2] Coll Life Sci, Dept Biotechnol, Nanchang 330031, Jiangxi, Peoples R China
来源：
MICROBIAL CELL FACTORIES | 2020年 / 19卷 / 01期
关键词：
L; lactis/pNZ8148-spax-HA; Cross protection; H5N1 cross-clade vaccine; INFLUENZA-A VIRUSES; VACCINE; SAFETY; IMMUNOGENICITY; DELIVERY; SUBUNIT; MUCOSAL; IMMUNIZATION; IMMUNITY;
D O I：
10.1186/s12934-020-01453-7
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Background: The highly pathogenic avian influenza (HPAI) H5N1 virus poses a potential threat to the poultry industry. The currently available avian influenza H5N1 vaccines for poultry are clade-specific. Therefore, an effective vaccine for preventing and controlling H5N1 viruses belonging to different clades needs to be developed. Results: RecombinantL. lactis/pNZ8148-Spax-HA was generated, and the influenza virus haemagglutinin (HA) protein of A/Vietnam/1203/2004 (H5N1) was displayed on the surface ofLactococcus lactis(L. lactis). Spax was used as an anchor protein. Chickens vaccinated orally with unadjuvantedL. lactis/pNZ8148-Spax-HA could produce significant humoral and mucosal responses and neutralizing activities against H5N1 viruses belonging to different clades. Importantly, unadjuvantedL. lactis/pNZ8148-Spax-HA conferred cross-clade protection against lethal challenge with different H5N1 viruses in the chicken model. Conclusion: This study provides insights into the cross-clade protection conferred by unadjuvantedL. lactis/pNZ8148-Spax-HA, and the results might help the establishment of a promising platform for the development of a safe and effective H5N1 cross-clade vaccine for poultry.
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页数：11
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