Listeria monocytogenes in Smoked Salmon and Other Smoked Fish at Retail in Italy: Frequency of Contamination and Strain Characterization in Products from Different Manufacturers

被引：1

作者：

Acciari, Vicdalia Aniela ^{[1
]}

Torresi, Marina ^{[1
]}

Iannetti, Luigi ^{[1
]}

Scattolini, Silvia ^{[1
]}

Pomilio, Francesco ^{[1
]}

Decastelli, Lucia ^{[2
]}

Colmegna, Silvia ^{[3
]}

Muliari, Riccardo ^{[4
]}

Bossu, Teresa ^{[5
]}

Proroga, Yolande ^{[6
]}

Montagna, Cosimo ^{[7
]}

Cardamone, Cinzia ^{[8
]}

Cogoni, Paola ^{[9
]}

Prencipe, Vincenza Annunziata ^{[1
]}

Migliorati, Giacomo ^{[1
]}

机构：

[1] Ist Zooprofilatt Sperimentale Abruzzo & Molise G, Natl Reference Lab Listeria Monocytogenes, I-64100 Campo Boario, Teramo, Italy

[2] Ist Zooprofilatt Sperimentale Piemonte Liguria &, Via Bologna 148, I-10154 Turin, Italy

[3] Ist Zooprofilatt Sperimentale Lombardia & Emilia, Via Bianchi 9, I-25124 Brescia, Italy

[4] Ist Zooprofilatt Sperimentale Venezie, Via Univ 10, I-35020 Legnaro, PD, Italy

[5] Ist Zooprofilatt Sperimentale Lazio & Toscana M A, Via Appia Nuova 1411, I-00178 Rome, Italy

[6] Ist Zooprofilatt Sperimentale Mezzogiorno, Via Salute 11, I-80055 Portici, NA, Italy

[7] Ist Zooprofilatt Sperimentale Puglia & Basilicata, Via Manfredonia 20, I-71121 Foggia, Italy

[8] Ist Zooprofilatt Sperimentale Sicilia A Mirri, Via G Marinuzzi 3, I-90129 Palermo, Italy

[9] Ist Zooprofilatt Sperimentale Sardegna G Pegreffi, Via Duca Abruzzi 8, I-07100 Sassari, Italy

来源：

JOURNAL OF FOOD PROTECTION | 2017年 / 80卷 / 02期

关键词：

Listeria monocytogenes; Manufacturer; Pulsed-field gel electrophoresis; Retail; Serotyping; Smoked salmon; FIELD GEL-ELECTROPHORESIS; PROCESSING ENVIRONMENT; PREVALENCE; SURVIVAL;

D O I：

10.4315/0362-028X.JFP-15-599

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Seven hundred seventy-eight samples of packaged smoked fish (774 smoked salmon and 4 smoked swordfish) on sale in Italy, from 50 different manufacturers located in 12 European Union countries, were purchased from the Italian market between May and December 2011. The surface temperatures of the samples on sale ranged from 0 to 130 degrees C (3.4 +/- 1.5 degrees C, mean +/- SD). Six hundred eighty (87.4%) of 778 samples were stored at <= 4 degrees C. One hundred fifty-seven samples (20.2%, 95% confidence interval 17.5 to 23.1%) were contaminated by Listeria monocytogenes, with 26 samples (3.3%, 95% confidence interval 2.3 to 4.8%) at levels >100 CFU/g. The maximum level of contamination was 1.3 X 10(6) CFU/g. The differences in the level of contamination of smoked fish between countries (chi(2) = 91.54, P < 0.05) and manufacturers (chi(2) = 193.22, P < 0.05) were significant. The frequency of detection for products from different manufacturing premises ranged from 0 to 76.9%. Serotyping by serological agglutination revealed that the main serotypes detected were 1/2a (65.3%) and 1/2b (22.4%). Pulsed-field gel electrophoresis typing with restriction enzymes AscI and ApaI yielded 36 pulsotypes from 144 isolates, clustering into 17 groups. Eight main pulsotypes accounted for 70.8% of the isolates. Three of the main pulsotypes were exclusively from products of a single manufacturer. In general, products from the same manufacturer showed genetic homogeneity, with one strongly prevalent pulsotype. Different manufacturers usually showed very different levels of contamination of the final product, confirming the importance of the management of process hygiene for controlling L. monocytogenes contamination.

引用

页码：271 / 278

页数：8

共 39 条

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