Fibroblast activation protein α identifies mesenchymal stromal cells from human bone marrow

被引：57

作者：

Bae, Sohyun ^{[1
]}

Park, Chae Woon ^{[1
]}

Son, Hye Kyung ^{[1
]}

Ju, Hye Kyung ^{[1
]}

Paik, Donggi ^{[1
]}

Jeon, Choon-Ju ^{[1
]}

Koh, Gou Young ^{[2
]}

Kim, Jaeseob ^{[2
]}

Kim, Hoeon ^{[1
]}

机构：

[1] Genexel Sein Inc, Biotherapeut Div, Taejon 305701, South Korea

[2] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon, South Korea

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 2008年 / 142卷 / 05期

关键词：

fibroblast activation protein alpha; mesenchymal stromal cells; surface protein marker;

D O I：

10.1111/j.1365-2141.2008.07241.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Mesenchymal stromal cells (MSCs) have gained widespread popularity in cell therapy, but their development into clinical products has been impeded by the scarcity of cell-specific markers. We previously explored transcriptome and membrane proteome of MSCs, from which fibroblast activation protein alpha (FAP) was recognized as a prime surface marker candidate. The present study showed that FAP was constitutively expressed on MSCs, but not on other cells. FAP immunoselection yielded homogeneous MSCs from cryopreserved bone marrow (BM). These results suggest that FAP serves as a surface protein marker that can singly define MSCs from BM and possibly from other sources.

引用

页码：827 / 830

页数：4

共 15 条

[1] Structural and kinetic analysis of the substrate specificity of human fibroblast activation protein α [J].

Aertgeerts, K ;

Levin, I ;

Shi, LH ;

Snell, GP ;

Jennings, A ;

Prasad, GS ;

Zhang, YM ;

Kraus, ML ;

Salakian, S ;

Sridhar, V ;

Wijnands, R ;

Tennant, MG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (20) :19441-19444

[2] The role of mesenchymal stem cells in haemopoiesis [J].

Dazzi, Francesco ;

Ramasamy, Rajesh ;

Glennie, Sarah ;

Jones, Simon P. ;

Roberts, Irene .

BLOOD REVIEWS, 2006, 20 (03) :161-171

[3]

Dolznig Helmut, 2005, Cancer Immun, V5, P10

[4] Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement [J].

Dominici, M. ;

Le Blanc, K. ;

Mueller, I. ;

Slaper-Cortenbach, I. ;

Marini, F. C. ;

Krause, D. S. ;

Deans, R. J. ;

Keating, A. ;

Prockop, D. J. ;

Horwitz, E. M. .

CYTOTHERAPY, 2006, 8 (04) :315-317

[5] SSEA-4 identifies mesenchymal stem cells from bone marrow [J].

Gang, Eun J. ;

Bosnakovski, Darko ;

Figueiredo, Camila A. ;

Visser, Jan W. ;

Perlingeiro, Rita C. R. .

BLOOD, 2007, 109 (04) :1743-1751

[6] Clarification of the nomenclature for MSC: The international society for cellular therapy position statement [J].

Horwitz, EM ;

Le Blanc, K ;

Dominici, M ;

Mueller, I ;

Slaper-Cortenbach, I ;

Marini, FC ;

Deans, RJ ;

Krause, DS ;

Keating, A .

CYTOTHERAPY, 2005, 7 (05) :393-395

[7] Differential gene expression profiling of human umbilical cord blood-derived mesenchymal stem cells by DNA microarray [J].

Jeong, JA ;

Hong, SH ;

Gang, EJ ;

Ahn, C ;

Hwang, SH ;

Yang, IH ;

Han, H ;

Kim, H .

STEM CELLS, 2005, 23 (04) :584-593

[8] Membrane proteomic analysis of human mesenchymal stromal cells during adipogenesis [J].

Jeong, Ju Ah ;

Ko, Kyung-Min ;

Park, Hyung Soon ;

Lee, Jinsook ;

Jang, Cholsoon ;

Jeon, Choon-Ju ;

Koh, Gou Young ;

Kim, Hoeon .

PROTEOMICS, 2007, 7 (22) :4181-4191

[9] Genome-wide differential gene expression profiling of human bone marrow stromal cells [J].

Jeong, Ju Ah ;

Ko, Kyung-Min ;

Bae, Sohyun ;

Jeon, Choon-Ju ;

Koh, Gou Young ;

Kim, Hoeon .

STEM CELLS, 2007, 25 (04) :994-1002

[10] Treatment of severe acute graft-versus-host disease with third party haploidentical mesenchymal stem cells [J].

Le Blanc, K ;

Rasmusson, I ;

Sundberg, B ;

Götherström, C ;

Hassan, M ;

Uzunel, M ;

Ringdén, O .

LANCET, 2004, 363 (9419) :1439-1441

← 1 2 →