Exosome-based antisense locked nucleic acid delivery for inhibition of type II collagen degradation in chondrocyte

被引：16

作者：

Jeong, Kyeongsoo ^{[1
]}

Jeong, Seunga ^{[1
]}

Kim, Jeong Ah ^{[3
]}

Rhee, Won Jong ^{[1
,2
]}

机构：

[1] Incheon Natl Univ, Dept Bioengn & Nanobioengn, Incheon 406772, South Korea

[2] Incheon Natl Univ, Div Bioengn, Incheon 406772, South Korea

[3] Korea Basic Sci Inst, Biomed Omics Grp, Cheongju 28119, South Korea

来源：

JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY | 2019年 / 74卷

基金：

新加坡国家研究基金会;

关键词：

MicroRNA; Osteoarthritis; Antisense locked nucleic acid; Exosome; Delivery vehicle; MESENCHYMAL STEM-CELLS; DRUG-DELIVERY; EXTRACELLULAR VESICLES; OSTEOARTHRITIS; CARTILAGE; MICRORNAS; EXPRESSION; SYSTEM; ELECTROPORATION; COMMUNICATION;

D O I：

10.1016/j.jiec.2019.02.017

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

MicroRNA-34a (miR-34a) suppression can block the progression of type II collagen decrease in chondrocytes for osteoarthritis (OA) treatment. Thus, exosomes, nano-sized extracellular vesicles, were developed as delivery vehicles for antisense miR-34a locked nucleic acid (LNA). As a result, antisense LNA-loaded exosomes exhibited no cytotoxic effects on the chondrocytes and inhibited IL-1 beta-mediated type II collagen decrease in inflammation-induced chondrocytes. Moreover, the suppression of chondrocyte growth was inhibited upon antisense LNA-loaded exosome treatment. In conclusion, the exosome-mediated antisense delivery approach was shown to be biocompatible and functional as a highly efficient OA treatment and could be applicable for treating other diseases. (C) 2019 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.

引用

页码：126 / 135

页数：10

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