Allele-specific polymerase chain reaction for detection of a mutation in the relax circular DNA and the covalently closed circular DNA of hepatitis B virus

被引：1
作者：
Pan, Wan-Long [1 ,2 ]
Hu, Jie-Li [1 ]
Fang, Yan [3 ]
Luo, Qiang [1 ]
Xu, Ge [1 ]
Xu, Lei [1 ]
Jing, Zhou-Hong [4 ]
Shan, Xue-Feng [1 ]
Zhu, Yan-Ling [5 ]
Huang, Ai-Long [1 ]
机构：
[1] Chongqing Med Univ, Minist Educ, Key Lab Mol Biol Infect Dis, Chongqing 400016, Peoples R China
[2] North Sichuan Med Coll, Dept Microbiol & Immunol, Nanchong 637000, Peoples R China
[3] Second Peoples Hosp Gansu Prov, Intens Care Unit, Lanzhou 730000, Peoples R China
[4] Chongqing Med Univ, Affiliated Hosp 2, Chongqing 400016, Peoples R China
[5] Third Peoples Hosp Chongqing, Dept Resp Med, Chongqing 400014, Peoples R China
来源：
JOURNAL OF VIROLOGICAL METHODS | 2013年 / 194卷 / 1-2期
关键词：
Point mutation; Allele-specific polymerase chain reaction; INFLUENZA HEMAGGLUTININ; HETEROLOGOUS STRAINS; IFN-GAMMA; H5N1; HA2; PROTECTION; ANTIBODIES; MICE; INFECTION; RESPONSES;
D O I：
10.1016/j.jviromet.2013.08.034
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The relax circle DNA (rcDNA) sequence and the covalently closed circle DNA (cccDNA) sequence in hepatitis B virus (HBV) are crucial regions for HBV infections. To analyze mutations in rcDNA and cccDNA, DNA sequencing is often used, although it is time-consuming and expensive. Herein, we report a simple, economic, albeit accurate allele-specific polymerase chain reaction (AS-PCR) to detect mutations in these regions of HBV. This method can be extensively used to screen for mutations at specific positions of HBV genome. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：277 / 288
页数：12
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共 30 条
  • [1] Current concepts - Avian influenza A (H5N1) infection in humans
    Beigel, H
    Farrar, H
    Han, AM
    Hayden, FG
    Hyer, R
    de Jong, MD
    Lochindarat, S
    Tien, NTK
    Hien, NT
    Hien, TT
    Nicoll, A
    Touch, S
    Yuen, KY
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2005, 353 (13) : 1374 - 1385
  • [2] Design of an HA2-based Escherichia coli expressed influenza immunogen that protects mice from pathogenic challenge
    Bommakanti, Gayathri
    Citron, Michael P.
    Hepler, Robert W.
    Callahan, Cheryl
    Heidecker, Gwendolyn J.
    Najar, Tariq Ahmad
    Lu, Xianghan
    Joyce, Joseph G.
    Shiver, John W.
    Casimiro, Danilo R.
    ter Meulen, Jan
    Liang, Xiaoping
    Varadarajan, Raghavan
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (31) : 13701 - 13706
  • [3] ANTIGENIC DRIFT IN INFLUENZA VIRUS-H3 HEMAGGLUTININ FROM 1968 TO 1980 - MULTIPLE EVOLUTIONARY PATHWAYS AND SEQUENTIAL AMINO-ACID CHANGES AT KEY ANTIGENIC SITES
    BOTH, GW
    SLEIGH, MJ
    COX, NJ
    KENDAL, AP
    [J]. JOURNAL OF VIROLOGY, 1983, 48 (01) : 52 - 60
  • [4] Human T helper (Th) cell lineage commitment is not directly linked to the secretion of IFN-γ or IL-4:: Characterization of Th cells isolated by FACS based on IFN-γ and IL-4 secretion
    Cao, WX
    Chen, YD
    Alkan, S
    Subramaniam, A
    Long, F
    Liu, H
    Diao, R
    Delohery, T
    McCormick, J
    Chen, R
    Ni, DH
    Wright, PS
    Zhang, X
    Busch, S
    Zilberstein, A
    [J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 2005, 35 (09) : 2709 - 2717
  • [5] Research and development of universal influenza vaccines
    Du, Lanying
    Zhou, Yusen
    Jiang, Shibo
    [J]. MICROBES AND INFECTION, 2010, 12 (04) : 280 - 286
  • [6] Antibody Recognition of a Highly Conserved Influenza Virus Epitope
    Ekiert, Damian C.
    Bhabha, Gira
    Elsliger, Marc-Andre
    Friesen, Robert H. E.
    Jongeneelen, Mandy
    Throsby, Mark
    Goudsmit, Jaap
    Wilson, Ian A.
    [J]. SCIENCE, 2009, 324 (5924) : 246 - 251
  • [7] Prospects for universal influenza virus vaccine
    Gerhard, W
    Mozdzanowska, K
    Zharikova, D
    [J]. EMERGING INFECTIOUS DISEASES, 2006, 12 (04) : 569 - 574
  • [8] INTER-PANDEMIC INFLUENZA IN HOUSTON AREA, 1974-76
    GLEZEN, WP
    COUCH, RB
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1978, 298 (11) : 587 - 592
  • [9] Antibodies induced by the HA2 glycopolypeptide of influenza virus haemagglutinin improve recovery from influenza A virus infection
    Gocnik, M.
    Fislova, T.
    Mucha, V.
    Sladkova, T.
    Russ, G.
    Kostolansky, F.
    Vareckova, E.
    [J]. JOURNAL OF GENERAL VIROLOGY, 2008, 89 : 958 - 967
  • [10] Universal antibodies against the highly conserved influenza fusion peptide cross-neutralize several subtypes of influenza A virus
    Hashem, Anwar M.
    Van Domselaar, Gary
    Li, Changgui
    Wang, Junzhi
    She, Yi-Min
    Cyr, Terry D.
    Sui, Jianhua
    He, Runtao
    Marasco, Wayne A.
    Li, Xuguang
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2010, 403 (02) : 247 - 251
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