Genome sequencing and comparative genomics of honey bee microsporidia, Nosema apis reveal novel insights into host-parasite interactions

被引：60

作者：

Chen, Yan Ping ^{[1
]}

Pettis, Jeffery S. ^{[1
]}

Zhao, Yan ^{[2
]}

Liu, Xinyue ^{[3
]}

Tallon, Luke J. ^{[3
]}

Sadzewicz, Lisa D. ^{[3
]}

Li, Renhua ^{[4
]}

Zheng, Huoqing ^{[5
]}

Huang, Shaokang ^{[6
]}

Zhang, Xuan ^{[7
]}

Hamilton, Michele C. ^{[1
]}

Pernal, Stephen F. ^{[8
]}

Melathopoulos, Andony P. ^{[8
]}

Yan, Xianghe ^{[9
]}

Evans, Jay D. ^{[1
]}

机构：

[1] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA

[2] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA

[3] Univ Maryland, Sch Med, Inst Genome Sci, Baltimore, MD 21202 USA

[4] Windber Res Inst, Windber, PA 15963 USA

[5] Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China

[6] Fujian Agr & Forestry Univ, Coll Bee Sci, Fuzhou 350002, Fujian, Peoples R China

[7] Yunnan Agr Univ, Eastern Bee Res Inst, Kunming 650201, Yunnan, Peoples R China

[8] Agr & Agri Food Canada, Beaverlodge Res Farm, Beaverlodge, AB, Canada

[9] Eastern Reg Res Ctr, Mol Characterizat Foodborne Pathogens Unit, Wyndmoor, PA 19038 USA

来源：

BMC GENOMICS | 2013年 / 14卷

关键词：

Microsporidia; Nosema; Honey bees; Genome; Comparative genomics; COLONY COLLAPSE; WEB SERVER; CERANAE; MELLIFERA; INFECTION; PATHOGEN; GENES; IDENTIFICATION; ANNOTATION; PREDICTION;

D O I：

10.1186/1471-2164-14-451

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: The microsporidia parasite Nosema contributes to the steep global decline of honey bees that are critical pollinators of food crops. There are two species of Nosema that have been found to infect honey bees, Nosema apis and N. ceranae. Genome sequencing of N. apis and comparative genome analysis with N. ceranae, a fully sequenced microsporidia species, reveal novel insights into host-parasite interactions underlying the parasite infections. Results: We applied the whole-genome shotgun sequencing approach to sequence and assemble the genome of N. apis which has an estimated size of 8.5 Mbp. We predicted 2,771 protein-coding genes and predicted the function of each putative protein using the Gene Ontology. The comparative genomic analysis led to identification of 1,356 orthologs that are conserved between the two Nosema species and genes that are unique characteristics of the individual species, thereby providing a list of virulence factors and new genetic tools for studying host-parasite interactions. We also identified a highly abundant motif in the upstream promoter regions of N. apis genes. This motif is also conserved in N. ceranae and other microsporidia species and likely plays a role in gene regulation across the microsporidia. Conclusions: The availability of the N. apis genome sequence is a significant addition to the rapidly expanding body of microsprodian genomic data which has been improving our understanding of eukaryotic genome diversity and evolution in a broad sense. The predicted virulent genes and transcriptional regulatory elements are potential targets for innovative therapeutics to break down the life cycle of the parasite.

引用

页数：16

共 80 条

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