Enantioseparation of β-methyl-substituted amino acids with cyclodextrins by capillary zone electrophoresis

被引：14

作者：

Ilisz, Istvan ^{[1
]}

Fodor, Gabor ^{[1
]}

Ivanyi, Robert ^{[2
]}

Szente, Lajos ^{[2
]}

Toth, Geza ^{[3
]}

Peter, Antal ^{[1
]}

机构：

[1] Univ Szeged, Dept Inorgan & Analyt Chem, H-6720 Szeged, Hungary

[2] Cyclolab R&D Lab, H-1097 Budapest, Hungary

[3] Biol Res Ctr, Inst Biochem, H-6726 Szeged, Hungary

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 875卷 / 01期

关键词：

Capillary zone electrophoresis; beta-Methyl-amino acids; Native and sulfated cyclodextrins; Cyclodextrin sulfates;

D O I：

10.1016/j.jchromb.2008.05.020

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct capillary zone electrophoretic methods were developed for the separation of the enantiomers of unnatural beta-methyl-amino acids such as erythro- and threo-beta-methylphenylalanine, beta-methyltyrosine, beta-methyltryptophan and beta-methyl-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid. Capillary zone electrophoresis was carried out using sulfopropylated-alpha-CD (SP2-alpha-CD), sulfopropylated-beta-CD (SP2-beta-CD) both with a degree of substitution of 2 moles/mole cyclodextrin, and sulfopropylated-beta-CD (SP4-beta-CD) with a degree of substitution of 4 moles/mole beta-cyclodextrin. The effects of selector and buffer concentrations, electrolyte pH and applied voltage were Studied on the separation efficiency. Varying the electrophoretic conditions with application of 20 kV, hydrodynamic injection, unmodified silica capillary, three different buffers (borate, phosphate and acetate) and modified cyclodextrins as chiral selectors all compounds investigated are nearly baseline resolved. The elution sequence was determined in most cases. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：273 / 279

页数：7

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