High throughput functional screening of an ion channel library for drug safety and efficacy

被引：13

作者：

Brown, Arthur M. ^{[1
]}

机构：

[1] ChanTest Corp, Cleveland, OH 44128 USA

来源：

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 2009年 / 38卷 / 03期

关键词：

Ion channel library; Automated patch clamp; Automated fluorescence; High throughput screening; Cardiac channel panel (TM);

D O I：

10.1007/s00249-008-0356-2

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Development of a large, representative library of ion channel-expressing cell lines is described. Validation on a full range of automated patch clamp and fluorescence illumination platforms is ongoing. Library "books" can be mixed and matched into channel panels according to tissue, therapeutic area and ion channel family. Unexpected results using a cardiac channel panel show that this panel may serve as a biomarker for cardiac risk assessment.

引用

页码：273 / 278

页数：6

共 7 条

[1]

Brown AM., 2000, PHARM NEWS, V7, P15

[2]

Kirsch Glenn E., 2004, Journal of Pharmacological and Toxicological Methods, V50, P93, DOI 10.1016/j.vascn.2004.06.003

[3]

LACERDA AE, 2005, NIH SCI SESS

[4] Alfuzosin delays cardiac repolarization by a novel mechanism [J].

Lacerda, Antonio E. ;

Kuryshev, Yuri A. ;

Chen, Yuan ;

Renganathan, Muthukrishnan ;

Eng, Heather ;

Danthi, Sanjay J. ;

Kramer, James W. ;

Yang, Tianen ;

Brown, Arthur M. .

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2008, 324 (02) :427-433

[5]

LODISH H, 2000, MOL BIOL

[6] VARIABILITY OF THE QT(C) INTERVAL - IMPACT ON DEFINING DRUG EFFECT AND LOW-FREQUENCY CARDIAC EVENT [J].

MORGANROTH, J ;

BROWN, AM ;

CRITZ, S ;

CRUMB, WJ ;

KUNZE, DL ;

LACERDA, AE ;

LOPEZ, H .

AMERICAN JOURNAL OF CARDIOLOGY, 1993, 72 (06) :B26-B31

[7] HERG, a primary human ventricular target of the nonsedating antihistamine terfenadine [J].

Roy, ML ;

Dumaine, R ;

Brown, AM .

CIRCULATION, 1996, 94 (04) :817-823

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