Moderate systemic therapeutic hypothermia is insufficient to protect blood-spinal cord barrier in spinal cord injury

被引:1
作者
Zhou, Rubing [1 ,2 ,3 ]
Li, Junzhao [2 ,3 ]
Wang, Ruideng [1 ]
Chen, Zhengyang [1 ]
Zhou, Fang [1 ]
机构
[1] Peking Univ Third Hosp, Dept Orthoped, Beijing, Peoples R China
[2] Peking Univ, Sch Basic Med Sci, Hlth Sci Ctr, Neurosci Res Inst, Beijing, Peoples R China
[3] Peking Univ, Sch Basic Med Sci, Hlth Sci Ctr, Dept Neurobiol, Beijing, Peoples R China
来源
FRONTIERS IN NEUROLOGY | 2022年 / 13卷
基金
中国国家自然科学基金;
关键词
spinal cord injury; hypothermia; blood-spinal cord barrier; two-photon microscopy; tight junctions; TRAUMATIC BRAIN-INJURY; INFLAMMATORY RESPONSE; TIGHT JUNCTIONS; PATHOPHYSIOLOGY; EXPRESSION; EXTRAVASATION; PERMEABILITY; TEMPERATURE; DISRUPTION; MECHANISMS;
D O I
10.3389/fneur.2022.1041099
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Blood-spinal cord barrier (BSCB) disruption is a pivotal event in spinal cord injury (SCI) that aggravates secondary injury but has no specific treatment. Previous reports have shown that systemic therapeutic hypothermia (TH) can protect the blood-brain barrier after brain injury. To verify whether a similar effect exists on the BSCB after SCI, moderate systemic TH at 32 degrees C was induced for 4 h on the mice with contusion-SCI. In vivo two-photon microscopy was utilized to dynamically monitor the BSCB leakage 1 h after SCI, combined with immunohistochemistry to detect BSCB leakage at 1 and 4 h after SCI. The BSCB leakage was not different between the normothermia (NT) and TH groups at both the in vivo and postmortem levels. The expression of endothelial tight junctions was not significantly different between the NT and TH groups 4 h after SCI, as detected by capillary western blotting. The structural damage of the BSCB was examined with immunofluorescence, but the occurrence of junctional gaps was not changed by TH 4 h after SCI. Our results have shown that moderate systemic TH induced for 4 h does not have a protective effect on the disrupted BSCB in early SCI. This treatment method has a low value and is not recommended for BSCB disruption therapy in early SCI.
引用
收藏
页数:10
相关论文
共 68 条
[1]   Hypothermia for Acute Spinal Cord Injury-A Review [J].
Ahmad, Faiz U. ;
Wang, Michael Y. ;
Levi, Allan D. .
WORLD NEUROSURGERY, 2014, 82 (1-2) :207-214
[2]   Traumatic spinal cord injury [J].
Ahuja, Christopher S. ;
Wilson, Jefferson R. ;
Nori, Satoshi ;
Kotter, Mark R. N. ;
Druschel, Claudia ;
Curt, Armin ;
Fehlings, Michael G. .
NATURE REVIEWS DISEASE PRIMERS, 2017, 3
[3]   Traumatic Spinal Cord Injury-Repair and Regeneration [J].
Ahuja, Christopher S. ;
Nori, Satoshi ;
Tetreault, Lindsay ;
Wilson, Jefferson ;
Kwon, Brian ;
Harrop, James ;
Choi, David ;
Fehlings, Michael G. .
NEUROSURGERY, 2017, 80 (03) :S9-S22
[4]   The Role of Therapeutic Hypothermia After Traumatic Spinal Cord Injury-A Systematic Review [J].
Alkabie, Samir ;
Boileau, Andrew J. .
WORLD NEUROSURGERY, 2016, 86 :432-449
[5]   THE BIOLOGY OF REGENERATION FAILURE AND SUCCESS AFTER SPINAL CORD INJURY [J].
Amanda Phuong Tran ;
Warren, Philippa Mary ;
Silver, Jerry .
PHYSIOLOGICAL REVIEWS, 2018, 98 (02) :881-917
[6]   Blood-Brain Barrier Permeability Is Regulated by Lipid Transport-Dependent Suppression of Caveolae-Mediated Transcytosis [J].
Andreone, Benjamin J. ;
Chow, Brian Wai ;
Tata, Aleksandra ;
Lacoste, Baptiste ;
Ben-Zvi, Ayal ;
Bullock, Kevin ;
Deik, Amy A. ;
Ginty, David D. ;
Clish, Clary B. ;
Gu, Chenghua .
NEURON, 2017, 94 (03) :581-+
[7]   The Blood-Spinal Cord Barrier: Morphology and Clinical Implications [J].
Bartanusz, Viktor ;
Jezova, Daniela ;
Alajajian, Betty ;
Digicaylioglu, Murat .
ANNALS OF NEUROLOGY, 2011, 70 (02) :194-206
[8]   Pericytes Control Key Neurovascular Functions and Neuronal Phenotype in the Adult Brain and during Brain Aging [J].
Bell, Robert D. ;
Winkler, Ethan A. ;
Sagare, Abhay P. ;
Singh, Itender ;
LaRue, Barb ;
Deane, Rashid ;
Zlokovic, Berislav V. .
NEURON, 2010, 68 (03) :409-427
[9]   UNDERSTANDING SECONDARY INJURY [J].
Ben Borgens, Richard ;
Liu-Snyder, Peishan .
QUARTERLY REVIEW OF BIOLOGY, 2012, 87 (02) :89-127
[10]   The history of therapeutic hypothermia and its use in neurosurgery [J].
Bohl, Michael A. ;
Martirosyan, Nikolay L. ;
Killeen, Zachary W. ;
Belykh, Evgenii ;
Zabramski, Joseph M. ;
Spetzler, Robert F. ;
Preul, Mark C. .
JOURNAL OF NEUROSURGERY, 2019, 130 (03) :1006-1020