Physiological characterization of Saprolegnia parasitica isolates from brown trout

被引：33

作者：

DieguezUribeondo, J ^{[1
]}

Cerenius, L ^{[1
]}

Soderhall, K ^{[1
]}

机构：

[1] UPPSALA UNIV, DEPT PHYSIOL BOT, S-75236 UPPSALA, SWEDEN

来源：

AQUACULTURE | 1996年 / 140卷 / 03期

关键词：

Saprolegnia; saprolegniasis; fungal disease; physiological adaptation; random amplification of polymorphic DNA (RAPD); fish diseases;

D O I：

10.1016/0044-8486(95)01176-5

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Saprolegnia parasitica has caused large mortalities in brown trout, Salmo trutta, in Spain. Several strains of Saprolegnia parasitica have been isolated from these epizootics and characterized regarding their physiological adaptation and genetic diversity. These isolates exhibit similar physiological characteristics, i.e. radial growth, zoospore mobility and germination after encystment and differ substantially from other strains of the Saprolegnia diclina-parasitica complex tested. Random amplification of polymorphic DNA polymerase chain reaction (RAPD-PCR) of total DNA revealed a genetic similarity of about 85-100% between Spanish trout isolates whereas only 20-45% similarity to other strains of the Saprolegnia diclina-parasitica complex was found. The results suggest that the Spanish isolates are closely related strains and that they might have originated from one clone.

引用

页码：247 / 257

页数：11

共 35 条

[31] DNA POLYMORPHISMS AMPLIFIED BY ARBITRARY PRIMERS ARE USEFUL AS GENETIC-MARKERS [J].

WILLIAMS, JGK ;

KUBELIK, AR ;

LIVAK, KJ ;

RAFALSKI, JA ;

TINGEY, SV .

NUCLEIC ACIDS RESEARCH, 1990, 18 (22) :6531-6535

[32] RAPID PRELIMINARY SCREENING OF SAPROLEGNIA ON FISH [J].

WILLOUGHBY, LG .

JOURNAL OF FISH DISEASES, 1985, 8 (05) :473-476

[33] SAPROLEGNIAS OF SALMONID FISH IN WINDERMERE - CRITICAL ANALYSIS [J].

WILLOUGHBY, LG .

JOURNAL OF FISH DISEASES, 1978, 1 (01) :51-67

[34]

WILLOUGHBY LG, 1984, NOVA HEDWIGIA, V39, P35

[35] EXPERIMENTAL STUDIES ON UPTAKE AND INTERACTION OF SPORES OF THE SAPROLEGNIA-DICLINA-PARASITICA COMPLEX WITH EXTERNAL MUCUS OF BROWN TROUT (SALMO, TRUTTA) [J].

WOOD, SE ;

WILLOUGHBY, LG ;

BEAKES, GW .

TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1988, 90 :63-73

← 1 2 3 4 →