Determination of serum alcohol using a disposable biosensor

被引：22

作者：

Luo, Peng ^{[2
]}

Liu, Yi ^{[3
]}

Xie, Guoming ^{[2
]}

Xiong, Xingliang

Deng, Shixiong ^{[4
]}

Song, Fangzhou ^{[1
]}

机构：

[1] Chongqing Med Univ, Coll Basic Med, Dept Biochem & Mol Biol, Chongqing 400016, Peoples R China

[2] Chongqing Med Univ, Dept Lab Med, Minist Educ, Key Lab Lab Med Diagnost, Chongqing 400016, Peoples R China

[3] Chongqing Med & Pharmaceut Coll, Dept Med Tech, Chongqing 400030, Peoples R China

[4] Chongqing Med Univ, Coll Basic Med, Inst Forens Med, Chongqing 400016, Peoples R China

来源：

FORENSIC SCIENCE INTERNATIONAL | 2008年 / 179卷 / 2-3期

关键词：

biosensor; scrum alcohol determination; drunk driving;

D O I：

10.1016/j.forsciint.2008.06.002

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

This paper presents a disposable biosensor to detect serum alcohol concentration. The proposed biosensor is fabricated using the cross-linking method to immobilize Alcohol Dehydrogenase (ADH) and Nicotinamide Adenine Dinucleotide (NAD(+)) on the screen-printed electrode modified with Meldola's Blue (MB) absorbed on Nafion. It is based on the electrocatalytic properties of MB as,in electron transfer mediator, which can catalyze the oxidation of NADH to NAD(+) at a low oxidizing potential, thus avoiding interferences due to the presence of oxidizable substances in the real serum samples. The biosensor response for alcohol is investigated in terms of pH, buffer solution, temperature and some interferents. It presents the good specificity, reproducibility, stability, accuracy and provides a fast response. The biosensor has been satisfactorily used for the measurement of serum alcohol. (C). 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：192 / 198

页数：7

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