PCR-RFLP analysis of mitochondrial DNA:: A reliable method for species identification

被引：188

作者：

Wolf, C

Rentsch, J

Hübner, P

机构：

[1] Univ Bern, Food Chem Lab, CH-3012 Bern, Switzerland

[2] Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1999年 / 47卷 / 04期

关键词：

species identification; game meat; PCR; RFLP; DNA sequence diversity; cyt b;

D O I：

10.1021/jf9808426

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A method for identification of game species has been developed on the basis of the amplification of a specific part of the mitochondrial genome (tRNA(Glu)/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several game species,the obtained 464-bp-long PCR products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphism (RFLP). Even closely related deer species could be distinguished by application of one or two RE. Natural polymorphisms of the target sequence within one species were examined for red deer (Cervus elaphus), and base pair substitutions were identified affecting the RFLP pattern.

引用

页码：1350 / 1355

页数：6

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