The 5′-end expressed sequence tags of Lotus japonicus

被引：1

作者：

Nomura, Mika ^{[1
]}

Van Tan, Dao ^{[1
]}

Arunothayanan, Hatthaya ^{[1
]}

Asamizu, Erika ^{[3
]}

Tabata, Satoshi ^{[3
]}

Kouchi, Hiroshi ^{[2
]}

Tajima, Shigeyuki ^{[1
]}

机构：

[1] Kagawa Univ, Fac Agr, Dept Life Sci, Miki, Kagawa 7610795, Japan

[2] Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058602, Japan

[3] Kazusa DNA Res Inst, Chiba 2920818, Japan

来源：

PLANT BIOTECHNOLOGY | 2008年 / 25卷 / 02期

关键词：

ESTs; Legume; Lotus japonicus; Nitrogen fixation;

D O I：

10.5511/plantbiotechnology.25.173

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

For a global analysis of gene expression during nodule tissue formation in a model legume, Lotus japonicus, a cDNA array was constructed using 18,144 non-redundant clone set deduced from 3'-end expressed sequence tags (EST, Kouchi et al. 2004). In the current study, to obtain peptide coding information from 5'-ends of clone, we generated 7,320 5' EST sequences. BLAST search against a protein database indicated that 65.5% of the ESTs showed significant similarity to registered sequences. We identified numerous of nodule-enhanced genes, including many involved in diverse cellular processes, suggesting that the EST resources generated in this study will be a useful tool for the identification of genes related to nodulation and symbiotic nitrogen fixation.

引用

页码：173 / 175

页数：3

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