TMC1 and TMC2 Are Components of the Mechanotransduction Channel in Hair Cells of the Mammalian Inner Ear

被引：317

作者：

Pan, Bifeng ^{[1
]}

Geleoc, Gwenaelle S. ^{[1
]}

Asai, Yukako ^{[1
]}

Horwitz, Geoffrey C. ^{[1
]}

Kurima, Kiyoto ^{[2
]}

Ishikawa, Kotaro ^{[2
]}

Kawashima, Yoshiyuki ^{[2
]}

Griffith, Andrew J. ^{[2
]}

Holt, Jeffrey R. ^{[1
]}

机构：

[1] Harvard Univ, Boston Childrens Hosp, Sch Med, Dept Otolaryngol,FM Kirby Neurobiol Ctr, Boston, MA 02115 USA

[2] Natl Inst Deafness & Other Commun Disorders, Otolaryngol Branch, NIH, Rockville, MD 20850 USA

来源：

NEURON | 2013年 / 79卷 / 03期

关键词：

MECHANOELECTRICAL TRANSDUCER CHANNELS; MECHANOSENSITIVE CHANNELS; FUNCTIONAL MATURATION; SENSORY TRANSDUCTION; MOUSE COCHLEA; C; ELEGANS; ADAPTATION; CALCIUM; DOMINANT; DEAFNESS;

D O I：

10.1016/j.neuron.2013.06.019

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Sensory transduction in auditory and vestibular hair cells requires expression of transmembrane channel-like (Tmc) 1 and 2 genes, but the function of these genes is unknown. To investigate the hypothesis that TMC1 and TMC2 proteins are components of the mechanosensitive ion channels that convert mechanical information into electrical signals, we recorded whole-cell and single-channel currents from mouse hair cells that expressed Tmc1, Tmc2, or mutant Tmc1. Cells that expressed Tmc2 had high calcium permeability and large single-channel currents, while cells with mutant Tmc1 had reduced calcium permeability and reduced single-channel currents. Cells that expressed Tmc1 and Tmc2 had a broad range of single-channel currents, suggesting multiple heteromeric assemblies of TMC subunits. The data demonstrate TMC1 and TMC2 are components of hair cell transduction channels and contribute to permeation properties. Gradients in TMC channel composition may also contribute to variation in sensory transduction along the tonotopic axis of the mammalian cochlea.

引用

页码：504 / 515

页数：12

共 41 条

[1] Production and characterization of improved adenovirus vectors with the E1, E2b, and E3 genes deleted [J].