Optimally chirped multimodal CARS microscopy based on a single Ti: sapphire oscillator

被引：171

作者：

Pegoraro, Adrian F. ^{[1
,2
]}

Ridsdale, Andrew ^{[2
]}

Moffatt, Douglas J. ^{[2
]}

Jia, Yiwei ^{[3
]}

Pezacki, John Paul ^{[2
]}

Stolow, Albert ^{[1
,2
]}

机构：

[1] Queens Univ, Dept Phys, Kingston, ON K7L 3N6, Canada

[2] Natl Res Council Canada, Steacie Inst Mol Sci, Ottawa, ON K1A 0R6, Canada

[3] Olympus America Inc, Sci Equipment Grp, Cent Valley, PA 18034 USA

来源：

OPTICS EXPRESS | 2009年 / 17卷 / 04期

关键词：

STOKES-RAMAN SCATTERING; HIGH-SPECTRAL-RESOLUTION; ZERO DISPERSION WAVELENGTHS; PHOTONIC CRYSTAL FIBER; SPECTROSCOPY; PULSES; FLUORESCENCE; GENERATION; LIPIDS;

D O I：

10.1364/OE.17.002984

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We demonstrate high performance coherent anti-Stokes Raman scattering ( CARS) microscopy of live cells and tissues with user-variable spectral resolution and broad Raman tunability ( 2500 - 4100 cm(-1)), using a femtosecond Ti: Sapphire pump and photonic crystal fiber output for the broadband synchronized Stokes pulse. Spectral chirp of the fs laser pulses was a user-variable parameter for optimization in a spectral focussing implementation of multimodal CARS microscopy. High signal-to-noise, high contrast multimodal imaging of live cells and tissues was achieved with pixel dwell times of 2-8 mu s and low laser powers (< 30 mW total). (c) 2009 Optical Society of America

引用

页码：2984 / 2996

页数：13

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