Rapid Identification of Staphylococcus Species Isolated from Food Samples by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

被引:25
作者
Kim, Eiseul [1 ,2 ]
Kim, Hyun-Joong [1 ,2 ]
Yang, Seung-Min [1 ,2 ]
Kim, Chang-Gyeom [3 ]
Choo, Dong-Won [3 ]
Kim, Hae-Yeong [1 ,2 ]
机构
[1] Kyung Hee Univ, Inst Life Sci & Resources, Yongin 17104, South Korea
[2] Kyung Hee Univ, Dept Food Sci & Biotechnol, Yongin 17104, South Korea
[3] Korea Polytech, Dept Bioinformat & Biosyst, Seongnam 13122, South Korea
关键词
MALDI-TOF MS; Staphylococcus; jeotgal; fermented food; identification; extraction method; 16S RIBOSOMAL-RNA; DESORPTION IONIZATION-TIME; COAGULASE-NEGATIVE STAPHYLOCOCCI; KOREAN FERMENTED FOODS; GENE; CLASSIFICATION; SYSTEM; MS; PERFORMANCE; STARTER;
D O I
10.4014/jmb.1901.01046
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Staphylococcus species have a ubiquitous habitat in a wide range of foods, thus the ability to identify staphylococci at the species level is critical in the food industry. In this study, we performed rapid identification of Staphylococcus species using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS was evaluated for the identification of Staphylococcus reference strains (n = 19) and isolates (n = 96) from various foods with consideration for the impact of sample preparation methods and incubation period. Additionally, the spectra of isolated Staphylococcus strains were analyzed using principal component analysis (PCA) and a main spectra profile (MSP)-based dendrogram. MALDI-TOF MS accurately identified Staphylococcus reference strains and isolated strains: the highest performance was by the EX method (83.3 similar to 89.5% accuracy) at species level identification (EDT, 70.3 similar to 78.9% accuracy; DT, less than 46.3 similar to 63.2% accuracy) of 24-h cultured colonies. Identification results at the genus level were 100% accurate at EDT, EX sample preparation and 24-h incubation time. On the other hand, the DT method showed relatively low identification accuracy in all extraction methods and incubation times. The analyzed spectra and MSP-based dendrogram showed that the isolated Staphylococcus strains were characterized at the species level. The performance analysis of MALDI-TOF MS shows the method has the potential ability to discriminate between Staphylococcus species from foods in Korea. This study provides valuable information that MALDI-TOF MS can be applied to monitor microbial populations and pathogenic bacteria in the food industry thereby contributing to food safety.
引用
收藏
页码:548 / 557
页数:10
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