Overexpression of polyphosphate kinase gene (ppk) increases bioinsecticide production by Bacillus thuringiensis

被引：13

作者：

Doruk, Tugrul ^{[1
]}

Avican, Ummehan ^{[1
]}

Camci, Irem Yalim ^{[1
]}

Gedik, Sedef Tunca ^{[1
]}

机构：

[1] Gebze Inst Technol, Dept Mol Biol & Genet, TR-41400 Gebze, Kocaeli, Turkey

来源：

MICROBIOLOGICAL RESEARCH | 2013年 / 168卷 / 04期

关键词：

Polyphosphate kinase; ppk; Bacillus thuringiensis; Endotoxin; Bioinsecticide; DELTA-ENDOTOXIN PRODUCTION; INORGANIC POLYPHOSPHATE; ESCHERICHIA-COLI; CRYSTAL PROTEIN; EXPRESSION; TOXIN; PHOSPHATE; ISRAELENSIS; MOTILITY; REMOVAL;

D O I：

10.1016/j.micres.2012.11.009

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Polyphosphate (polyP), synthesized by polyp kinase (PPK) using the terminal phosphate of ATP as substrate, performs important functions in every living cell. The present work reports on the relationship between polyp metabolism and bioinsecticide production in Bacillus thuringiensis subsp. israelensis (Bti). The ppk gene of Bti was cloned into vector pHT315 and the effect of its overexpression on endotoxin production was determined. Endotoxin production by the recombinant strain was found to be consistently higher than that by the wild type strain and the strain that carried the empty plasmid. The toxicity of the recombinant mutant strain (LC50 5.8 +/- 0.6 ng ml(-1)) against late 2nd instar Culex quinquefasciatus was about 7.7 times higher than that of Bti (LC50 44.9 +/- 7 ng ml(-1)). To our knowledge this is the first reported study which relates polyp metabolism with bioinsecticide biosynthesis. (C) 2012 Elsevier GmbH. All rights reserved.

引用

页码：199 / 203

页数：5

共 40 条

[1] HOW DOES BACILLUS-THURINGIENSIS PRODUCE SO MUCH INSECTICIDAL CRYSTAL PROTEIN [J].

AGAISSE, H ;

LERECLUS, D .

JOURNAL OF BACTERIOLOGY, 1995, 177 (21) :6027-6032

[2]

Ahn A, 1990, J BIOL CHEM, V265, P11734

[3]

AKIYAMA M, 1993, J BIOL CHEM, V268, P633

[4] CONSTRUCTION OF CLONING VECTORS FOR BACILLUS-THURINGIENSIS [J].

ARANTES, O ;

LERECLUS, D .

GENE, 1991, 108 (01) :115-119

[5] Inorganic phosphate regulates CryIVA protoxin expression in Bacillus thuringiensis israelensis [J].

Banerjee-Bhatnagar, N .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 262 (02) :359-364

[6]

Bravo A, 1996, MOL GEN GENET, V250, P734

[7] Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control [J].

Bravo, Alejandra ;

Gill, Sarjeet S. ;

Soberon, Mario .

TOXICON, 2007, 49 (04) :423-435

[8] Inorganic polyphosphate in the origin and survival of species [J].

Brown, MRW ;

Kornberg, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (46) :16085-16087

[9] Large Crystal Toxin Formation in Chromosomally Engineered Bacillus thuringiensis subsp aizawai Due to σE Accumulation [J].

Buasri, Wasin ;

Panbangred, Watanalai .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2012, 78 (06) :1682-1691

[10] Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins [J].

Crickmore, N ;

Zeigler, DR ;

Feitelson, J ;

Schnepf, E ;

Van Rie, J ;

Lereclus, D ;

Baum, J ;

Dean, DH .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (03) :807-+

← 1 2 3 4 →