A genome-wide integrated analysis of lncRNA-mRNA in melanocytes from white and brown skin hair boer goats (Capra aegagrus hircus)

被引:4
作者
Kai-yuan, Ji [1 ,2 ]
Yi-Wei, Zhao [1 ]
Ru-jun, Wen [1 ]
Khan, Ibrar Muhammad [1 ,3 ]
Yun-hai, Zhang [1 ,4 ]
机构
[1] Anhui Agr Univ, Coll Anim Sci & Technol, Anhui Key Lab Genet Resources Protect & Biol Breed, Hefei, Peoples R China
[2] Anhui Agr Univ, Coll Anim Sci & Technol, Anhui Prov Key Lab Vet Pathobiol & Dis Control, Hefei, Peoples R China
[3] Fuyang Normal Univ, Sch Biol & Food Engn, Anhui Prov Key Lab Embryo Dev & Reprod Regulat, Anhui Prov Key Lab Environm Hormone & Reprod, Fuyang, Peoples R China
[4] Anhui Agr Univ, Linquan Comprehens Expt Stn, Linquan, Peoples R China
关键词
lncRNA; melanocyte; Capra hircus; genome-wide analysis; mRNA; EXPRESSION; PROLIFERATION; TRANSCRIPTION; STRINGTIE; GROWTH; CELLS;
D O I
10.3389/fvets.2022.1009174
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Long noncoding RNAs (lncRNAs) are involved in many biological processes and have been extensively researched. Nonetheless, literature focusing on the roles of lncRNA in melanocytes is limited. Melanocytes are located in the basal layer of the epidermis and determine the color of an animal's skin and hair by producing melanin. The mechanisms of melanogenesis remain unclear. Here, melanocytes from Boer goat skins were successfully isolated and verified using morphological observation, dopamine staining, silver ammonia staining, and immunohistochemical staining in vitro. Phenotypic testing revealed that melanocytes isolated from goat skins with white and brown hairs showed significant differences in proliferation, migration, and melanogenesis (*P < 0.01). RNA sequencing was performed with the isolated melanocytes, and through bioinformatic analysis, several candidate lncRNAs and mRNAs involved in stage-specific melanogenesis were identified. Functional enrichment analysis indicated that miRNA precursors and cis-regulatory effects of lncRNAs were deeply dissected using the function prediction software. Multiple lncRNA-mRNA networks were presumed to be involved in melanocyte migration, proliferation, and melanogenesis based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation. This research provided novel bioinformatic insights into the roles of lncRNAs in mammalian pigmentation.
引用
收藏
页数:11
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