Application of the consolidated species concept to identify the causal agent of strawberry anthracnose in Iran and initial molecular dating of the Colletotrichum acutatum species complex

被引:23
作者
Karimi, Kaivan [1 ,2 ]
Ahari, Asadollah Babai [1 ]
Arzanlou, Mahdi [1 ]
Amini, Jahanshir [3 ]
Pertot, Ilaria [2 ]
Rota-Stabelli, Omar [2 ]
机构
[1] Tabriz Univ, Dept Plant Protect, Tabriz, Iran
[2] Fdn Edmund Mach, Res & Innovat Ctr, Dept Sustainable Agroecosyst & Bioresources, San Michele All Adige, Italy
[3] Kurdistan Univ, Dept Plant Protect, Sanandaj, Iran
关键词
Colletotrichum nymphaeae; Introduction; Molecular clock; Multi-gene approach; Kurdistan; GENETIC DIVERSITY; SEQUENCE-ANALYSIS; SPP; NOV; GLOEOSPORIOIDES; IDENTIFICATION; PHYLOGENY; PATHOGEN; STRAINS; COMB;
D O I
10.1007/s10658-016-1009-4
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Anthracnose caused by Colletotrichum spp. is one of main strawberry diseases worldwide. The disease recently arrived in Iran and become epidemic in the Kurdistan province. A combination of morphological, molecular and pathogen-host interaction approaches (referred to the consolidated species concept) were applied to isolates from symptomatic strawberry tissues collected in Iran. Multi-gene phylogenetic analysis based on ITS-rDNA, beta-tubulin (TUB2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) identified the species as the S5 clade of Colletotrichum nymphaeae. No significant intraspecies variation was observed in terms of morphology and pathogenicity. No significant genetic variation was observed among the isolates using inter simple sequence repeat analysis (ISSR) and primer pair combination of ERIC1/BOX and ERIC2/BOX fingerprinting markers. Based on the results of this study, it can be postulated that the C. nymphaeae population in Iran established itself from a single origin due to a founder effect. More generally, molecular dating based on relaxed clocks indicates co-radiation of C. nymphaeae S5 and strawberry plants and suggests high reciprocal specificity between the host and pathogen.
引用
收藏
页码:375 / 387
页数:13
相关论文
共 56 条
[1]   Analysis of population structure of Rosellinia necatrix on Cyperus esculentus by mycelial compatibility and inter-simple sequence repeats (ISSR) [J].
Armengol, J. ;
Vicent, A. ;
Leon, M. ;
Berbegal, M. ;
Abad-Campos, P. ;
Garcia-Jimenez, J. .
PLANT PATHOLOGY, 2010, 59 (01) :179-185
[2]  
Arzanlou Mahdi, 2015, Journal of Plant Protection Research, V55, P198, DOI 10.1515/jppr-2015-0027
[3]  
Ayoubi N., 2013, 1 IR MYC C 3 5 SEP 2
[4]   Molecular Diversity of Anthracnose Pathogen Populations Associated with UK Strawberry Production Suggests Multiple Introductions of Three Different Colletotrichum Species [J].
Baroncelli, Riccardo ;
Zapparata, Antonio ;
Sarrocco, Sabrina ;
Sukno, Serenella A. ;
Lane, Charles R. ;
Thon, Michael R. ;
Vannacci, Giovanni ;
Holub, Eric ;
Sreenivasaprasad, Surapareddy .
PLOS ONE, 2015, 10 (06)
[5]   CHARACTERISTICS OF COLLETOTRICHUM FROM PEACH, APPLE, PECAN, AND OTHER HOSTS [J].
BERNSTEIN, B ;
ZEHR, EI ;
DEAN, RA ;
SHABI, E .
PLANT DISEASE, 1995, 79 (05) :478-482
[6]   Molecular characterization of slow-growing orange and Key lime anthracnose strains of Colletotrichum from citrus as C-acutatum [J].
Brown, AE ;
Sreenivasaprasad, S ;
Timmer, LW .
PHYTOPATHOLOGY, 1996, 86 (05) :523-527
[7]  
Bruns T., 1990, PCR PROTOCOLS GUIDE, P482
[8]   Molecular characterization of Colletotrichum strains derived from strawberry [J].
Buddie, AG ;
Martínez-Culebras, P ;
Bridge, PD ;
García, MD ;
Querol, A ;
Cannon, PF ;
Monte, E .
MYCOLOGICAL RESEARCH, 1999, 103 :385-394
[9]  
Cai L, 2009, FUNGAL DIVERS, V39, P183
[10]   Strawberry anthracnose:: Histoplathology of Colletotrichum acutatum and C-fragariae [J].
Curry, KJ ;
Abril, M ;
Avant, JB ;
Smith, BJ .
PHYTOPATHOLOGY, 2002, 92 (10) :1055-1063