Greater filamin C, GSK3α, and GSK3β serine phosphorylation in insulin-stimulated isolated skeletal muscles of calorie restricted 24 month-old rats

被引：12

作者：

Sequea, Donel A. ^{[1
]}

Sharma, Naveen ^{[2
]}

Arias, Edward B. ^{[2
]}

Cartee, Gregory D. ^{[1
,2
,3
]}

机构：

[1] Univ Michigan, Dept Mol & Integrat Physiol, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Sch Kinesiol, Muscle Biol Lab, Ann Arbor, MI 48109 USA

[3] Univ Michigan, Inst Gerontol, Ann Arbor, MI 48109 USA

来源：

MECHANISMS OF AGEING AND DEVELOPMENT | 2013年 / 134卷 / 1-2期

关键词：

Ageing; Insulin signaling; Muscle fiber type; Dietary restriction; GLUCOSE-UPTAKE; AKT2; PHOSPHORYLATION; TBC1D1; TWITCH;

D O I：

10.1016/j.mad.2012.12.002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Moderate calorie restriction (CR) can improve insulin-stimulated Akt phosphorylation and glucose uptake in muscles from 24 month-old rats, but the specific Akt substrates linking CR-effects on Akt to glucose uptake and other cellular processes are uncertain. We probed CR's influence on site-specific phosphorylation of five Akt substrates (AS160(Ser588), TBC1D1(Thr596), FLNc(Ser2213), GSK3 alpha(Ser21), and GSK3 beta(Ser9)) in predominantly fast-twitch (epitrochlearis) and predominantly slow-twitch (soleus) muscles. We observed no CR-effect on phosphorylation of AS160(Ser588) or TBC1D1(Thr596), but there was a CR-induced increase in insulin-stimulated FLNc(Ser2213,) GSK3 alpha(Ser21), and GSK3 beta(Ser9) phosphorylation for both muscles. These results indicate that CR does not uniformly affect insulin-mediated phosphorylation of Akt substrates in fast- or slow-twitch muscles from 24 month-old rats. (c) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：60 / 63

页数：4

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