Bacillus subtilis attachment to Aspergillus niger hyphae results in mutually altered metabolism

被引:108
作者
Benoit, Isabelle [1 ,2 ,3 ,4 ]
van den Esker, Marielle H. [5 ]
Patyshakuliyeva, Aleksandrina [1 ,3 ]
Mattern, Derek J. [6 ,7 ]
Blei, Felix [8 ]
Zhou, Miaomiao [1 ,3 ]
Dijksterhuis, Jan [1 ]
Brakhage, Axel A. [6 ,7 ]
Kuipers, Oscar P. [4 ,5 ]
de Vries, Ronald P. [1 ,3 ,4 ]
Kovacs, Akos T. [5 ,8 ]
机构
[1] CBS KNAW Fungal Biodivers Ctr, Fungal Physiol, Utrecht, Netherlands
[2] Univ Utrecht, Microbiol, Utrecht, Netherlands
[3] Univ Utrecht, Fungal Mol Physiol, Utrecht, Netherlands
[4] Netherlands Org Sci Res, Netherlands Genom Initiat, Kluyver Ctr Genom Ind Fermentat, Delft, Netherlands
[5] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Mol Genet, Groningen, Netherlands
[6] Leibniz Inst Nat Prod Res & Infect Biol HKI, Mol & Appl Microbiol Dept, Jena, Germany
[7] Univ Jena, Dept Microbiol & Mol Biol, Jena, Germany
[8] Univ Jena, Inst Microbiol, Terr Biofilms Grp, Jena, Germany
关键词
GENE-EXPRESSION; CANDIDA-ALBICANS; TRANSCRIPTOME ANALYSIS; BIOFILM FORMATION; BACTERIAL; SPORULATION; NIDULANS; GROWTH; CLUSTERS; PATHWAY;
D O I
10.1111/1462-2920.12564
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Interaction between microbes affects the growth, metabolism and differentiation of members of the microbial community. While direct and indirect competition, like antagonism and nutrient consumption have a negative effect on the interacting members of the population, microbes have also evolved in nature not only to fight, but in some cases to adapt to or support each other, while increasing the fitness of the community. The presence of bacteria and fungi in soil results in various interactions including mutualism. Bacilli attach to the plant root and form complex communities in the rhizosphere. Bacillus subtilis, when grown in the presence of Aspergillus niger, interacts similarly with the fungus, by attaching and growing on the hyphae. Based on data obtained in a dual transcriptome experiment, we suggest that both fungi and bacteria alter their metabolism during this interaction. Interestingly, the transcription of genes related to the antifungal and putative antibacterial defence mechanism of B.subtilis and A.niger, respectively, are decreased upon attachment of bacteria to the mycelia. Analysis of the culture supernatant suggests that surfactin production by B.subtilis was reduced when the bacterium was co-cultivated with the fungus. Our experiments provide new insights into the interaction between a bacterium and a fungus.
引用
收藏
页码:2099 / 2113
页数:15
相关论文
共 81 条
[41]   Structurally diverse natural products that cause potassium leakage trigger multicellularity in Bacillus subtilis [J].
Lopez, Daniel ;
Fischbach, Michael A. ;
Chu, Frances ;
Losick, Richard ;
Kolter, Roberto .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (01) :280-285
[42]   Transcriptome analysis of Lactococcus lactis in coculture with Saccharomyces cerevisiae [J].
Maligoy, Mathieu ;
Mercade, Myriam ;
Cocaign-Bousquet, Muriel ;
Loubiere, Pascal .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (02) :485-494
[43]   antiSMASH: rapid identification, annotation and analysis of secondary metabolite biosynthesis gene clusters in bacterial and fungal genome sequences [J].
Medema, Marnix H. ;
Blin, Kai ;
Cimermancic, Peter ;
de Jager, Victor ;
Zakrzewski, Piotr ;
Fischbach, Michael A. ;
Weber, Tilmann ;
Takano, Eriko ;
Breitling, Rainer .
NUCLEIC ACIDS RESEARCH, 2011, 39 :W339-W346
[44]   Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger [J].
Mela, Francesca ;
Fritsche, Kathrin ;
de Boer, Wietse ;
van Veen, Johannes A. ;
de Graaff, Leo H. ;
van den Berg, Marlies ;
Leveau, Johan H. J. .
ISME JOURNAL, 2011, 5 (09) :1494-1504
[45]   The structure of dimethylallyl tryptophan synthase reveals a common architecture of aromatic prenyltransferases in fungi and bacteria [J].
Metzger, Ute ;
Schall, Christoph ;
Zocher, Georg ;
Unsoeld, Inge ;
Stec, Edyta ;
Li, Shu-Ming ;
Heide, Lutz ;
Stehle, Thilo .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (34) :14309-14314
[46]   From environmental signals to regulators: Modulation of biofilm development in Gram-positive bacteria [J].
Mhatre, Eisha ;
Monterrosa, Ramses Gallegos ;
Kovacs, Akos T. .
JOURNAL OF BASIC MICROBIOLOGY, 2014, 54 (07) :616-632
[47]   Distinct Roles of ComK1 and ComK2 in Gene Regulation in Bacillus cereus [J].
Mironczuk, Aleksandra M. ;
Manu, Amagoia ;
Kuipers, Oscar P. ;
Kovacs, Akos T. .
PLOS ONE, 2011, 6 (07)
[48]  
Mohammadipour M, 2009, CAN J MICROBIOL, V55, P395, DOI [10.1139/W08-141, 10.1139/w08-141]
[49]   Antifungal mechanisms by which a novel Pseudomonas aeruginosa phenazine toxin kills Candida albicans in biofilms [J].
Morales, Diana K. ;
Jacobs, Nicholas J. ;
Rajamani, Sathish ;
Krishnamurthy, Malathy ;
Cubillos-Ruiz, Juan R. ;
Hogan, Deborah A. .
MOLECULAR MICROBIOLOGY, 2010, 78 (06) :1379-1392
[50]   A proteomics approach to study synergistic and antagonistic interactions of the fungal-bacterial consortium Fusarium oxysporum wild-type MSA 35 [J].
Moretti, Marino ;
Grunau, Alexander ;
Minerdi, Daniela ;
Gehrig, Peter ;
Roschitzki, Bernd ;
Eberl, Leo ;
Garibaldi, Angelo ;
Gullino, Maria Lodovica ;
Riedel, Kathrin .
PROTEOMICS, 2010, 10 (18) :3292-3320