A reagentless signal-on architecture for electronic, aptamer-based sensors via target-induced strand displacement

被引：476

作者：

Xiao, Y

Piorek, BD

Plaxco, KW ^{[1
]}

Heeger, AJ

机构：

[1] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Dept Phys, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Dept Mat, Santa Barbara, CA 93106 USA

[3] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Inst Polymers & Organ Solids, Santa Barbara, CA 93106 USA

[4] Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 51期

关键词：

D O I：

10.1021/ja056555h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Thrombin binding stabilizes the alternative G-quadruplex conformation of the aptamer, liberating the methylene blue (MB)-tagged oligonucleotide to produce a flexible, single-stranded DNA element. This allows the MB tag to collide with the gold electrode surface, producing a readily detectable Faradaic current at thrombin concentrations as low as ∼3 nM. Copyright © 2005 American Chemical Society.

引用

收藏

页码：17990 / 17991

页数：2

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