Candida rugosa Lipase Immobilized onto Acid-Functionalized Multi-walled Carbon Nanotubes for Sustainable Production of Methyl Oleate

被引：29

作者：

Marzuki, Nur Haziqah Che ^{[1
]}

Mahat, Naji Arafat ^{[1
]}

Huyop, Fahrul ^{[2
]}

Buang, Nor Aziah ^{[1
]}

Wahab, Roswanira Abdul ^{[1
]}

机构：

[1] Univ Teknol Malaysia, Fac Sci, Dept Chem, Johor Baharu 81310, Johor, Malaysia

[2] Univ Teknol Malaysia, Fac Biosci & Med Engn, Dept Biotechnol & Med Engn, Johor Baharu 81310, Johor, Malaysia

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2015年 / 177卷 / 04期

关键词：

Multi-walled carbon nanotubes; Candida rugosa lipase; Immobilization; Esterification; Methyl oleate; CATALYZED ESTERIFICATION; ENZYMATIC ESTERIFICATION; REACTION PARAMETERS; GERANYL PROPIONATE; ORGANIC-SOLVENT; STABILITY; BIODIESEL; MEDIA; ESTERS;

D O I：

10.1007/s12010-015-1791-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chemical production of methyl oleate using chemically synthesized fatty acid alcohols and other toxic chemicals may lead to significant environmental hazards to mankind. Being a highly valuable fatty acid replacement raw material in oleochemical industry, the mass production of methyl oleate via environmentally favorable processes is of concern. In this context, an alternative technique utilizing Candida rugosa lipase (CRL) physically adsorbed on multi-walled carbon nanotubes (MWCNTs) has been suggested. In this study, the acid-functionalized MWCNTs prepared using a mixture of HNO3 and H2SO4 (1:3 v/v) was used as support for immobilizing CRL onto MWCNTs (CRL-MWCNTs) as biocatalysts. Enzymatic esterification was performed and the efficiency of CRL-MWCNTs was evaluated against the free CRL under varying conditions, viz. temperature, molar ratio of acid/alcohol, solvent log P, and enzyme loading. The CRL-MWCNTs resulted in 30-110 % improvement in the production of methyl oleate over the free CRL. The CRL-MWCNTs attained its highest yield (84.17 %) at 50 A degrees C, molar ratio of acid/alcohol of 1:3, 3 mg/mL of enzyme loading, and iso-octane (log P 4.5) as solvent. Consequently, physical adsorption of CRL onto acid-functionalized MWCNTs has improved the activity and stability of CRL and hence provides an environmentally friendly means for the production of methyl oleate.

引用

页码：967 / 984

页数：18

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