Keel bone fractures induce a depressive-like state in laying hens

被引:42
作者
Armstrong, E. A. [1 ,2 ]
Rufener, C. [3 ,4 ]
Toscano, M. J. [4 ]
Eastham, J. E. [1 ,2 ]
Guy, J. H. [1 ,5 ]
Sandilands, V [6 ]
Boswell, T. [1 ,5 ]
Smulders, T., V [1 ,2 ]
机构
[1] Newcastle Univ, Ctr Behav & Evolut, Newcastle Upon Tyne, Tyne & Wear, England
[2] Newcastle Univ, Biosci Inst, Newcastle Upon Tyne, Tyne & Wear, England
[3] Univ Calif Davis, Dept Anim Sci, Davis, CA USA
[4] Univ Bern, Ctr Proper Housing Poultry & Rabbits ZTHZ, Zollikofen, Switzerland
[5] Newcastle Univ, Sch Nat & Environm Sci, Newcastle Upon Tyne, Tyne & Wear, England
[6] SRUC, Dept Agr Hort & Engn Sci, Edinburgh, Midlothian, Scotland
基金
英国生物技术与生命科学研究理事会;
关键词
ADULT HIPPOCAMPAL NEUROGENESIS; CELL-PROLIFERATION; EGG-PRODUCTION; EXERCISE INCREASES; BEHAVIORAL NEEDS; COLUMBA-LIVIA; STRESS; MOBILITY; NEURONS; DOUBLECORTIN;
D O I
10.1038/s41598-020-59940-1
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In commercial flocks of laying hens, keel bone fractures (KBFs) are prevalent and associated with behavioural indicators of pain. However, whether their impact is severe enough to induce a depressive-like state of chronic stress is unknown. As chronic stress downregulates adult hippocampal neurogenesis (AHN) in mammals and birds, we employ this measure as a neural biomarker of subjective welfare state. Radiographs obtained longitudinally from Lohmann Brown laying hens housed in a commercial multi-tier aviary were used to score the severity of naturally-occurring KBFs between the ages of 21-62 weeks. Individual birds' transitions between aviary zones were also recorded. Focal hens with severe KBFs at 3-4 weeks prior to sampling (n = 15) had lower densities of immature doublecortinpositive (DCX+) multipolar and bipolar neurons in the hippocampal formation than focal hens with minimal fractures (n = 9). KBF severity scores at this time also negatively predicted DCX+ cell numbers on an individual level, while hens that acquired fractures earlier in their lives had fewer DCX+ neurons in the caudal hippocampal formation. Activity levels 3-4 weeks prior to sampling were not associated with AHN. KBFs thus lead to a negative affective state lasting at least 3-4 weeks, and management steps to reduce their occurrence are likely to have significant welfare benefits.
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页数:14
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