Serum Albumin-Alginate Microparticles Prepared by Transacylation: Relationship between Physicochemical, Structural and Functional Properties

被引：23

作者：

Hadef, Imane ^{[1
,2
]}

Roge, Barbara ^{[2
]}

Edwards-Levy, Florence ^{[1
]}

机构：

[1] Univ Reims, Inst Chim Mol Reims, CNRS UMR 7312, UFR Pharm, F-51096 Reims, France

[2] Univ Reims, Inst Chim Mol Reims, CNRS UMR 7312, UFR Sci Exactes & Nat, F-51687 Reims, France

来源：

BIOMACROMOLECULES | 2015年 / 16卷 / 08期

关键词：

PROTEIN RELEASE; RHEOLOGICAL PROPERTIES; FRACTAL ANALYSIS; MICROSPHERES; MICROENCAPSULATION; MICROCAPSULES; LYSOZYME; ENCAPSULATION; PARAMETERS; MEMBRANE;

D O I：

10.1021/acs.biomac.5b00536

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Our laboratory develops a method of microencapsulation using a transacylation reaction in a water-in-oil (W/O) emulsion. The method is based on the creation of amide bonds between free amine functions of a protein (human serum albumin (HSA)) and ester groups of propylene glycol alginate (PGA) in the inner aqueous phase after alkalization. The aim of this work is to study the influence of physicochemical properties of HSA-PGA mixtures on microparticle characteristics. Microparticles were prepared varying the concentrations of PGA and HSA, then characterized (inner structure, size, swelling rate, release kinetics). PGA and each polymer mixture used in the microencapsulation procedure were examined in order to elucidate the mechanism of microstructure formation. It was found that the morphology and functional properties of HSA-alginate microparticles were related to the two polymer concentrations in the aqueous solution. Actually, the polymer concentration variations led to physicochemical changes, which affected the microparticle structure and functional properties.

引用

页码：2296 / 2307

页数：12

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