Characterization of tissue-specific mbu-3 gene expression in the mouse central nervous system

被引：1

作者：

Lee, Chae Jin ^{[1
]}

Cho, Eun Young ^{[1
]}

Kim, Sun Jung ^{[1
]}

机构：

[1] Dongguk Univ, Dept Life Sci, Seoul 100715, South Korea

来源：

BMB REPORTS | 2008年 / 41卷 / 12期

关键词：

Digital differential display; In situ hybridization; LRR domain; Mouse brain; Unigene;

D O I：

10.5483/BMBRep.2008.41.12.875

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mbu-3 is a novel mouse brain unigene that was identified by digital differential display. In this study, expression of the gene was chased through developmental stages and the protein product was identified in the brain. The cDNA sequence was 3,995-bp long and contained an ORF of 745 AA. Database searches revealed that the chicken SST273 gene containing LRR- and Ig-domain was an mbu-3 orthologue. Tissue specificity for the gene was examined in embryos and in brains at post-natal and adult stages. Dudring the embryonic stages, mbu-3 was localized to the central nervous system in the brain and spinal cord. In the early post-natal stages, the gene was evenly expressed in the brain. However, with aging, expression was confined to specific regions, particularly the hippocampus. The protein was approximately 95 kDa as determined by Western blot analysis of brain extracts. [BMB reports 2008; 41(12): 875-880]

引用

页码：875 / 880

页数：6

共 23 条

[1]

Alting-Mees Michelle A, 2006, Methods Mol Biol, V313, P97

[2] A SAGE-based screen for genes expressed in sub-populations of neurons in the mouse dorsal root ganglion [J].

Bourane, Steeve ;

Mechaly, Ilana ;

Venteo, Stephanie ;

Garces, Alain ;

Fichard, Agnes ;

Valmier, Jean ;

Carroll, Patrick .

BMC NEUROSCIENCE, 2007, 8

[3] Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation [J].

Brandenberger, R ;

Wei, H ;

Zhang, S ;

Lei, S ;

Murage, J ;

Fisk, GJ ;

Li, Y ;

Xu, CH ;

Fang, R ;

Guegler, K ;

Rao, MS ;

Mandalam, R ;

Lebkowski, J ;

Stanton, LW .

NATURE BIOTECHNOLOGY, 2004, 22 (06) :707-716

[4] A transcriptome database for astrocytes, neurons, and oligodendrocytes: A new resource for understanding brain development and function [J].

Cahoy, John D. ;

Emery, Ben ;

Kaushal, Amit ;

Foo, Lynette C. ;

Zamanian, Jennifer L. ;

Christopherson, Karen S. ;

Xing, Yi ;

Lubischer, Jane L. ;

Krieg, Paul A. ;

Krupenko, Sergey A. ;

Thompson, Wesley J. ;

Barres, Ben A. .

JOURNAL OF NEUROSCIENCE, 2008, 28 (01) :264-278

[5] Ensembl 2008 [J].

Flicek, P. ;

Aken, B. L. ;

Beal, K. ;

Ballester, B. ;

Caccamo, M. ;

Chen, Y. ;

Clarke, L. ;

Coates, G. ;

Cunningham, F. ;

Cutts, T. ;

Down, T. ;

Dyer, S. C. ;

Eyre, T. ;

Fitzgerald, S. ;

Fernandez-Banet, J. ;

Graf, S. ;

Haider, S. ;

Hammond, M. ;

Holland, R. ;

Howe, K. L. ;

Howe, K. ;

Johnson, N. ;

Jenkinson, A. ;

Kahari, A. ;

Keefe, D. ;

Kokocinski, F. ;

Kulesha, E. ;

Lawson, D. ;

Longden, I. ;

Megy, K. ;

Meidl, P. ;

Overduin, B. ;

Parker, A. ;

Pritchard, B. ;

Prlic, A. ;

Rice, S. ;

Rios, D. ;

Schuster, M. ;

Sealy, I. ;

Slater, G. ;

Smedley, D. ;

Spudich, G. ;

Trevanion, S. ;

Vilella, A. J. ;

Vogel, J. ;

White, S. ;

Wood, M. ;

Birney, E. ;

Cox, T. ;

Curwen, V. .

NUCLEIC ACIDS RESEARCH, 2008, 36 :D707-D714

[6] Analysis of nuclear localization signals using a green fluorescent protein-fusion protein library [J].

Fujii, G ;

Tsuchiya, R ;

Ezoe, E ;

Hirohashi, S .

EXPERIMENTAL CELL RESEARCH, 1999, 251 (02) :299-306

[7] The LRR and Ig domain-containing membrane protein SST273 is expressed on motoneurons [J].

Gejima, R ;

Okafuji, T ;

Tanaka, H .

GENE EXPRESSION PATTERNS, 2006, 6 (03) :235-240

[8] Systematic gene expression mapping clusters nuclear receptors according to their function in the brain [J].

Gofflot, Francoise ;

Chartoire, Nathalie ;

Vasseur, Laurent ;

Heikkinen, Sami ;

Dembele, Doulaye ;

Le Merrer, Julie ;

Auwerx, Johan .

CELL, 2007, 131 (02) :405-418

[9] Analysis of cag-8:: A novel poly(Q)-encoding gene in the mouse brain [J].

Ji, Jin Woo ;

Yang, Hye Lim ;

Kim, Sun Jung .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 346 (04) :1254-1260

[10] Development and application of a rat ovarian gene expression database [J].

Jo, MS ;

Gieske, MC ;

Payne, CE ;

Wheeler-Price, SE ;

Gieske, JB ;

Ignatius, IV ;

Curry, TE ;

Ko, CY .

ENDOCRINOLOGY, 2004, 145 (11) :5384-5396

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