Thrombin immobilization to Poly(Methacrylicacid) graft polymerized PET and PAN fabrics

被引：5

作者：

Akkaya, Alper ^{[1
]}

Pazarlioglu, Nurdan Kasikara ^{[1
]}

机构：

[1] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey

来源：

FIBERS AND POLYMERS | 2013年 / 14卷 / 03期

关键词：

Thrombin; Graft polymerization; Immobilization; Poly(ethylene terephthalate) fabric; Poly(acylonitrile) fabric; REACTIVE MEMBRANES; OXIDASE; SURFACE; POLY(ETHYLENE-TEREPHTHALATE); ADSORPTION; COPOLYMERS; TYROSINASE; KINETICS; ALCOHOL; ENZYMES;

D O I：

10.1007/s12221-013-0358-2

中图分类号：

TB3 [工程材料学]; TS1 [纺织工业、染整工业];

学科分类号：

0805 ; 080502 ; 0821 ;

摘要：

Poly(ethylene terephthalate) and poly(acrylonitrile) fabrics are the most produced synthetic fabrics in the world. Their production and usage increase at medical textile. There is no functional group in their structure for enzyme immobilization. Hence, they are not inclined to react. However, functional groups could be added to their structure using graft polymerization. In this study, methacrylic acid was graft polymerized to poly(ethylene terephthalate) and poly(acylonitrile) fabrics. Characterization of modified fabrics was carried out and thrombin was immobilized to poly(methacrylic acid) graft polymerized poly(ethylene terephthalate) and poly(acylonitrile) fabrics using 1-Ethyl-3-(3-dimetylaminopropyl)-carbodiimide. Optimization studies were also performed for the immobilization of thrombin. Thrombin immobilized poly(methacrylic acid) graft polymerized poly(ethylene terephtalate) and poly(acrylonitrile) fabrics were reduced recalcification time 30 % and 25 %, respectively. It is the first time, an enzyme was immobilized to fabric and its in vitro applications were performed. Thrombin has not been immobilized to synthetic fabric, yet.

引用

页码：358 / 364

页数：7

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