Identification and genetic diversity analysis of Memecylon species using ISSR, RAPD and Gene-based DNA barcoding tools

被引：5
作者：
Ramasetty, Bharathi Tumkur [1 ]
Bajpe, Shrisha Naik [1 ]
Kadappa, Sampath Kumara Kigga [1 ]
Saini, Ramesh Kumar [2 ]
Basavaraju, Shashibhushan Nittur [3 ]
Ramachandra, Kini Kukkundoor [1 ]
Sripathy, Prakash Harishchandra [1 ]
机构：
[1] Univ Mysore, Dept Studies Biotechnol, Mysore 570006, Karnataka, India
[2] Konkuk Univ, Coll Life & Environm Sci, Dept Bioresource & Food Sci, Seoul 143701, South Korea
[3] GKVK, Dept Crop Physiol, Bangalore, Karnataka, India
来源：
ELECTRONIC JOURNAL OF BIOTECHNOLOGY | 2016年 / 24卷
关键词：
atpF-atpH; Memecylon species; ndh; psbA-trnH; rpoC1; 5s; PCR based techniques; Molecular phylogenetic analysis; Molecular phylogenesis; DNA markers; MELASTOMATACEAE; MARKERS; CLASSIFICATION; PHYLOGENY; EVOLUTION; INDIA;
D O I：
10.1016/j.ejbt.2016.09.001
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Background: Memecylon species are commonly used in Indian ethnomedical practices. The accurate identification is vital to enhance the drug's efficacy and biosafety. In the present study, PCR based techniques like RAPD, ISSR and DNA barcoding regions, such as 5s, psbA-trnH, rpoC1, ndh and atpF-atpH, were used to authenticate and analyze the diversity of five Memecylon species collected from Western Ghats of India. Results: Phylogenetic analysis clearly distinguished Memecylon malabaricum from Memecylon wightii and Memecylon umbellatum from Memecylon edule and clades formed are in accordance with morphological keys. In the RAPD and ISSR analyses, 27 accessions representing five Memecylon species were distinctly separated into three different clades. M. malabaricum and M. wightii grouped together and M. umbellatum, M. edule and Memecylon talbotianum grouped in the same clade with high Jaccard dissimilarity coefficient and bootstrap support between each node, indicating that these grouped species are phylogenetically similar. Conclusion: Data from the present study reveals that chloroplast psbA-trnH region could be used as a potential candidate region for identifying Memecylon species, and ISSR marker system could be used for estimating genetic diversity since it has high percent polymorphism compared to RAPD marker. (C) 2016 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.
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页码：1 / 8
页数：8
相关论文
共 34 条
  • [1] Almedia MR, 1998, FLORA MAHARASHTRA
  • [2] [Anonymous], 2004, FOREST TREES S INDIA
  • [3] Apte GS, 2006, CURR SCI INDIA, V91, P1634
  • [4] Awano T, 2009, P NATL ACAD SCI USA, V106, P2794, DOI [10.1073/pnas.0905845106, 10.1073/pnas.0812297106]
  • [5] Ayyappan N, 2012, CHECK LIST, V8, P280
  • [6] Determination of inter- and intra-species genetic relationships among six Eucalyptus species based on inter-simple sequence repeats (ISSR)
    Balasaravanan, T
    Chezhian, P
    Kamalakannan, R
    Ghosh, A
    Yasodha, R
    Varghese, M
    Gurumurthi, K
    [J]. TREE PHYSIOLOGY, 2005, 25 (10) : 1295 - 1302
  • [7] Bharathi RT, 2016, INT J PHARM PHARM SC, V8, P1
  • [8] Bharathi TR, 2015, RES J PHARM BIOL CHE, V6, P1280
  • [9] Bharathi TR, 2014, INT J PHYTOPHARMACY, V4, P148
  • [10] Bhat KG, 2014, FLORA S KANARA UDUPI
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