Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate

被引：96
作者：
Kweon, CH [1 ]
Kwon, BJ
Lee, JG
Kwon, GO
Kang, YB
机构：
[1] Natl Vet Res & Quarantine Serv, Anyang 480, South Korea
[2] Dangjin Livestock Cooperat, Dangjin 549, South Korea
来源：
VACCINE | 1999年 / 17卷 / 20-21期
关键词：
porcine epidemic diarrhea virus; attenuation; vaccine candidate;
D O I：
10.1016/S0264-410X(99)00059-6
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The field isolate of porcine epidemic diarrhea virus (PEDV) was serially passaged in Vero cells. The cell passaged PEDV, designated KPEDV-9, was tested for its pathogenicity in the neonatal pigs, immunogenicity and safety in the pregnant sows. The result indicated that KPEDV-9 at the 93rd passage revealed reduced pathogenicity in the neonatal Digs. Pregnant sows inoculated with the attenuated virus showed increased immune responses by ELISA. In addition, delivered piglets were protected from challenge of wild type PEDV. The safety test in pregnant sows indicated that all inoculated animals farrowed the average numbers of litters of piglets. The results of this study supported that the attenuated virus derived from serial passage could be applied as vaccine for protecting suckling piglets against PEDV infection. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：2546 / 2553
页数：8
相关论文
共 50 条
[31]   Susceptibility of Mice to Porcine Epidemic Diarrhea Virus [J].
Kamau, N. A. ;
Park, J. Y. ;
Park, J. E. ;
Hyun, B. H. ;
Yang, D. K. ;
Song, J. Y. ;
Shin, H. J. .
JOURNAL OF ANIMAL AND VETERINARY ADVANCES, 2010, 9 (24) :3114-3116
[32]   Immunoprophylactic effect of chicken egg yolk immunoglobulin (Ig Y) against porcine epidemic diarrhea virus (PEDV) in piglets [J].
Kweon, CH ;
Kwon, BJ ;
Woo, SR ;
Kim, JM ;
Woo, GH ;
Son, DH ;
Hur, W ;
Lee, YS .
JOURNAL OF VETERINARY MEDICAL SCIENCE, 2000, 62 (09) :961-964
[33]   Sequence and phylogenetic analyses of the M and N genes of porcine epidemic diarrhea virus (PEDV) strains in Anhui Province, China [J].
Sun, P. ;
Gu, C. Y. ;
Ding, Y. Y. ;
Wei, J. H. ;
Yin, Z. J. ;
Geng, Z. Y. ;
Li, Y. .
GENETICS AND MOLECULAR RESEARCH, 2015, 14 (04) :13403-13413
[34]   The role of ORF3 accessory protein in replication of cell-adapted porcine epidemic diarrhea virus (PEDV) [J].
Phonphimon Wongthida ;
Benjamas Liwnaree ;
Nanchaya Wanasen ;
Jaraspim Narkpuk ;
Anan Jongkaewwattana .
Archives of Virology, 2017, 162 :2553-2563
[35]   Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) samples from field cases in Fujian, China [J].
Chen, Xi ;
Yang, Jinxian ;
Yu, Fusong ;
Ge, Junqing ;
Lin, Tianlong ;
Song, Tieying .
VIRUS GENES, 2012, 45 (03) :499-507
[36]   Emerging Highly Virulent Porcine Epidemic Diarrhea Virus: Molecular Mechanisms of Attenuation and Rational Design of Live Attenuated Vaccines [J].
Hou, Yixuan ;
Wang, Qiuhong .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (21)
[37]   Limited shedding of an S-InDel strain of porcine epidemic diarrhea virus (PEDV) in semen and questions regarding the infectivity of the detected virus [J].
Gallien, Sarah ;
Moro, Angelique ;
Lediguerher, Gerald ;
Catinot, Virginie ;
Paboeuf, Frederic ;
Bigault, Lionel ;
Gauger, Phillip C. ;
Pozzi, Nathalie ;
Berri, Mustapha ;
Authie, Edith ;
Rose, Nicolas ;
Grasland, Beatrice .
VETERINARY MICROBIOLOGY, 2019, 228 :20-25
[38]   Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) samples from field cases in Fujian, China [J].
Xi Chen ;
Jinxian Yang ;
Fusong Yu ;
Junqing Ge ;
Tianlong Lin ;
Tieying Song .
Virus Genes, 2012, 45 :499-507
[39]   Porcine Epidemic Diarrhea Virus (PEDV) ORF3 Interactome Reveals Inhibition of Virus Replication by Cellular VPS36 Protein [J].
Kaewborisuth, Challika ;
Yingchutrakul, Yodying ;
Roytrakul, Sittiruk ;
Jongkaewwattana, Anan .
VIRUSES-BASEL, 2019, 11 (04)
[40]   Efficacy of a commercial porcine epidemic diarrhea virus vaccine at reducing duration of viral shedding in gilts [J].
Brown, Justin ;
Rademacher, Chris ;
Baker, Samuel ;
Poonsuk, Korakrit ;
Cheng, Ting-Yu ;
Skoland, Kristin ;
Canning, Paisley ;
Forseth, Anna ;
Karriker, Locke .
JOURNAL OF SWINE HEALTH AND PRODUCTION, 2019, 27 (05) :256-264
12345