Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate

被引：96
作者：
Kweon, CH [1 ]
Kwon, BJ
Lee, JG
Kwon, GO
Kang, YB
机构：
[1] Natl Vet Res & Quarantine Serv, Anyang 480, South Korea
[2] Dangjin Livestock Cooperat, Dangjin 549, South Korea
来源：
VACCINE | 1999年 / 17卷 / 20-21期
关键词：
porcine epidemic diarrhea virus; attenuation; vaccine candidate;
D O I：
10.1016/S0264-410X(99)00059-6
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The field isolate of porcine epidemic diarrhea virus (PEDV) was serially passaged in Vero cells. The cell passaged PEDV, designated KPEDV-9, was tested for its pathogenicity in the neonatal pigs, immunogenicity and safety in the pregnant sows. The result indicated that KPEDV-9 at the 93rd passage revealed reduced pathogenicity in the neonatal Digs. Pregnant sows inoculated with the attenuated virus showed increased immune responses by ELISA. In addition, delivered piglets were protected from challenge of wild type PEDV. The safety test in pregnant sows indicated that all inoculated animals farrowed the average numbers of litters of piglets. The results of this study supported that the attenuated virus derived from serial passage could be applied as vaccine for protecting suckling piglets against PEDV infection. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：2546 / 2553
页数：8
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