Bioactive enzyme-metal composites: The entrapment of acid phosphatase within gold and silver

被引：41

作者：

Ben-Knaz, Racheli ^{[1
]}

Avnir, David ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

来源：

BIOMATERIALS | 2009年 / 30卷 / 07期

基金：

以色列科学基金会;

关键词：

Bioactivity; Composite; Enzyme; Acid phosphatase; Gold; Silver; SOL-GEL MATERIALS; ORGANIC-MOLECULES; AMINO-ACIDS; POLYMERS; PROTEINS; BIOENCAPSULATION; COPPER;

D O I：

10.1016/j.biomaterials.2008.11.026

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

This paper is concerned with the entrapment of an enzyme within an aggregated metallic matrix and the development of a bioactive enzyme-metal composite. Whereas the use of organic polymers and metal oxides for the preparation of enzymatically active materials is well developed, the third principle enzyme-material combination, namely protein-metal bulk, has not yet been reported. A new methodology for the entrapment of organic molecules and polymers within metals has been employed for the preparation of bioactive acid phosphatase@gold and acid phosphatase@silver, according to which room temperature reduction of the metal cation is carried out in the presence of the enzyme to be entrapped. Protectability of the entrapped enzyme against harsh conditions is shown: the acidic enzyme is kept alive under basic conditions. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1263 / 1267

页数：5

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