Profiling circulating tumour cells and other biomarkers of invasive cancers

被引：198

作者：

Poudineh, Mahla ^{[1
]}

Sargent, Edward H. ^{[2
]}

Pantel, Klaus ^{[3
]}

Kelley, Shana O. ^{[1
,4
,5
]}

机构：

[1] Univ Toronto, Leslie Dan Fac Pharm, Dept Pharmaceut Sci, Toronto, ON, Canada

[2] Univ Toronto, Dept Elect & Comp Engn, Fac Engn, Toronto, ON, Canada

[3] Univ Med Ctr Hamburg Eppendorf, Inst Tumor Biol, Hamburg, Germany

[4] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON, Canada

[5] Univ Toronto, Dept Biochem, Fac Med, Toronto, ON, Canada

来源：

NATURE BIOMEDICAL ENGINEERING | 2018年 / 2卷 / 02期

基金：

加拿大健康研究院; 欧洲研究理事会; 美国国家卫生研究院;

关键词：

METASTATIC BREAST-CANCER; EPITHELIAL-MESENCHYMAL PLASTICITY; LABEL-FREE; LIQUID BIOPSIES; ENDOTHELIAL BARRIER; MICROFLUIDIC DEVICE; RARE CELLS; DNA; EXOSOMES; CAPTURE;

D O I：

10.1038/s41551-018-0190-5

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

During cancer progression, many tumours shed circulating tumour cells (CTCs) and other biomarkers into the bloodstream. The analysis of CTCs offers the prospect of collecting a liquid biopsy from a patient's blood to predict and monitor therapeutic responses and tumour recurrence. In this Review, we discuss progress towards the isolation and recovery of bulk CTCs from whole blood samples for the identification of cells with high metastatic potential. We provide an overview of the techniques that initially pointed to the clinical significance of CTCs and describe the key requirements for clinical applications of CTC analysis. We also summarize recent advances that permit the functional and biochemical phenotypes of CTCs to be characterized, and discuss the potential roles of these CTC characteristics in the formation of metastatic lesions. Moreover, we discuss the use of circulating tumour DNA and exosomes as markers for early cancer diagnosis and for the monitoring of cancer progression. Next-generation technologies and biomarkers for invasive cancers should allow for the unequivocal determination of the metastatic potential of CTCs, and for the meaningful analysis of circulating tumour DNA and exosomes.

引用

页码：72 / 84

页数：13

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