Butane and propane oxidation by engineered cytochrome P450cam

被引：58

作者：

Bell, SG ^{[1
]}

Stevenson, JA ^{[1
]}

Boyd, HD ^{[1
]}

Campbell, S ^{[1
]}

Riddle, AD ^{[1
]}

Orton, EL ^{[1
]}

Wong, LL ^{[1
]}

机构：

[1] Univ Oxford, Dept Chem, Inorgan Chem Lab, Oxford OX1 3QR, England

来源：

CHEMICAL COMMUNICATIONS | 2002年 / 05期

关键词：

D O I：

10.1039/b110957j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The haem monooxygenase cytochrome P450(cam) has been engineered to oxidise the gaseous alkanes butane and propane to butan-2-ol and propan-2-ol, respectively, by the use of bulky amino acid substitutions to reduce the volume of the substrate pocket and thus improve the enzyme-substrate fit: the F87W/Y96F/T101L/V247L mutant oxidises butane with a turnover rate of 750 min(-1) and 95% yield based on NADH consumed while the wild-type enzyme has an activity of 0.4 min(-1) with 4% yield.

引用

页码：490 / 491

页数：2

共 15 条

[11] A 175-PSEC MOLECULAR-DYNAMICS SIMULATION OF CAMPHOR-BOUND CYTOCHROME-P-450CAM [J].

PAULSEN, MD ;

ORNSTEIN, RL .

PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1991, 11 (03) :184-204

[12] HIGH-RESOLUTION CRYSTAL-STRUCTURE OF CYTOCHROME-P450CAM [J].

POULOS, TL ;

FINZEL, BC ;

HOWARD, AJ .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 195 (03) :687-700

[13] The catalytic oxidation of linear and branched alkanes by cytochrome P450(cam) [J].

Stevenson, JA ;

Westlake, ACG ;

Whittock, C ;

Wong, LL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (50) :12846-12847

[14] Engineering molecular recognition in alkane oxidation catalysed by cytochrome P450cam [J].

Stevenson, JA ;

Bearpark, JK ;

Wong, LL .

NEW JOURNAL OF CHEMISTRY, 1998, 22 (06) :551-552

[15]

Wong LL, 1997, STRUCT BOND, V88, P175

← 1 2 →