Butane and propane oxidation by engineered cytochrome P450cam

被引：58

作者：

Bell, SG ^{[1
]}

Stevenson, JA ^{[1
]}

Boyd, HD ^{[1
]}

Campbell, S ^{[1
]}

Riddle, AD ^{[1
]}

Orton, EL ^{[1
]}

Wong, LL ^{[1
]}

机构：

[1] Univ Oxford, Dept Chem, Inorgan Chem Lab, Oxford OX1 3QR, England

来源：

CHEMICAL COMMUNICATIONS | 2002年 / 05期

关键词：

D O I：

10.1039/b110957j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The haem monooxygenase cytochrome P450(cam) has been engineered to oxidise the gaseous alkanes butane and propane to butan-2-ol and propan-2-ol, respectively, by the use of bulky amino acid substitutions to reduce the volume of the substrate pocket and thus improve the enzyme-substrate fit: the F87W/Y96F/T101L/V247L mutant oxidises butane with a turnover rate of 750 min(-1) and 95% yield based on NADH consumed while the wild-type enzyme has an activity of 0.4 min(-1) with 4% yield.

引用

页码：490 / 491

页数：2

共 15 条

[1] Engineering the haem monooxygenase cytochrome P450cam for monoterpene oxidation [J].

Bell, SG ;

Sowden, RJ ;

Wong, LL .

CHEMICAL COMMUNICATIONS, 2001, (07) :635-636

[2]

Dalton H., 1993, P65

[3]

de Montellano P.R. Ortiz., 1995, CYTOCHROME P450 STRU

[4]

Gunsalus I C, 1978, Methods Enzymol, V52, P166

[5] The oxidation of polychlorinated benzenes by genetically engineered cytochrome P450cam:: potential applications in bioremediation [J].

Jones, JP ;

O'Hare, EJ ;

Wong, LL .

CHEMICAL COMMUNICATIONS, 2000, (03) :247-248

[6] MICROBIAL-DEGRADATION OF HYDROCARBONS IN THE ENVIRONMENT [J].

LEAHY, JG ;

COLWELL, RR .

MICROBIOLOGICAL REVIEWS, 1990, 54 (03) :305-315

[7] BIOCHEMISTRY OF THE SOLUBLE METHANE MONOOXYGENASE [J].

LIPSCOMB, JD .

ANNUAL REVIEW OF MICROBIOLOGY, 1994, 48 :371-399

[8] MOLECULAR RECOGNITION IN CYTOCHROME-P-450 - MECHANISM FOR THE CONTROL OF UNCOUPLING REACTIONS [J].

LOIDA, PJ ;

SLIGAR, SG .

BIOCHEMISTRY, 1993, 32 (43) :11530-11538

[9]

Mueller Ernest J., 1995, P83

[10] The catalytic activity of cytochrome P450(cam) towards styrene oxidation is increased by site-specific mutagenesis [J].

Nickerson, DP ;

HarfordCross, CF ;

Fulcher, SR ;

Wong, LL .

FEBS LETTERS, 1997, 405 (02) :153-156

← 1 2 →