Two Novel Potent -Amylase Inhibitors from the Family of Acarviostatins Isolated from the Culture of Streptomyces coelicoflavus ZG0656

被引：7

作者：

Geng, Peng ^{[1
]}

Sun, Ting ^{[2
]}

Zhong, Qiping ^{[1
]}

Li, Xiaoxia ^{[1
]}

Shi, Liying ^{[1
]}

Bai, Fang ^{[3
]}

Bai, Gang ^{[3
]}

机构：

[1] Tianjin Med Univ, Sch Basic Med Sci, Dept Microbiol, Tianjin State Lab Cellular & Mol Immunol, Tianjin 300070, Peoples R China

[2] Tianjin Med Univ, Basic Med Res Ctr, Tianjin 300070, Peoples R China

[3] Nankai Univ, Coll Pharmaceut Sci, Tianjin 300071, Peoples R China

来源：

CHEMISTRY & BIODIVERSITY | 2013年 / 10卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Streptomyces coelicoflavus; Acarviostatins III0(1) and III23; -Amylase; Inhibitiors; Saccharide hydrolase; IONIZATION MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; ALPHA-AMYLASE; LUTEOGRISEUS; AMINOOLIGOSACCHARIDES; METABOLITES; ACARBOSE;

D O I：

10.1002/cbdv.201100451

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two novel aminooligosaccharides were separated from the culture filtrate of Streptomyces coelicoflavus ZG0656. Their chemical structures were determined by acidic hydrolysis, electrospray-ionization tandem mass spectrometry (ESI-MS/MS), and NMR spectroscopy. The compounds were named acarviostatins III0(1) and III23 according to the nomenclature of this group of metabolites. The two novel acarviostatins were both mixed noncompetitive inhibitors of porcine pancreatic -amylase (PPA). The inhibition constants (Ki) for acarviostatins III0(1) and III23 were 0.009 and 0.026M, respectively, 151 and 52 times more potent than acarbose.

引用

页码：452 / 459

页数：8